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Plasmid#27271DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsGSTExpressionBacterialMutationS327APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-1m
Plasmid#13551DepositorInsertFLIP-E intramol A207W (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationA207W, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-GFP-iRFP670
Plasmid#111814PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
GFP
iRFP670
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-oChD-citrine
Plasmid#50976PurposeAAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITRDepositorInsertoChD-citrine
UseAAVTagscitrineExpressionMutationChD gene is mammalian codon optimizedPromoterhuman synapsin promoterAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBW211J109-Amp32C
Plasmid#78646PurposepSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ109-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0016
Plasmid#117526PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.0DepositorInsertSp-eCas9 1.0 (with stop codon)
UseCRISPRTagsExpressionMutationK810A, K1003A, R1060APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSY091
Plasmid#110068PurposeBacterial expression of L4DepositorInsertL4
UseTagsExpressionMutationAT-swapped mutantPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSY075
Plasmid#110067PurposeBacterial expression of L2DepositorInsertL2
UseTagsExpressionBacterialMutationAT-swapped mutantPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mEGFP-C
Plasmid#113749PurposeGateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP
UseCRISPR; Gateway entry vectorTagsmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-C
Plasmid#113755PurposeGateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-C
Plasmid#113757PurposeGateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD8
Plasmid#112820PurposepSMF2 with 8 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD10
Plasmid#112821PurposepSMF2 with 10 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pANY1
Plasmid#110949PurposeUniversal mini-vector for efficient cloning of DNA and expression of protein in E. coli.DepositorInsertccd B cassette
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
ptp4a3_L (TAL 3000)
Plasmid#35992DepositorInsertTAL array targeting ptp4a3 (ptp4a3a Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_CMV53-sfGFP-CMV_dsRed Exp
Plasmid#117402PurposesfGFP expressed from hypoxia-inducible CMV53 promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_miniTK-sfGFP-CMV_dsRed Exp
Plasmid#117404PurposesfGFP expressed from hypoxia-inducible miniTK promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTUT6
Plasmid#60043PurposeMammalian expression of FLAG-tagged mouse TUT6DepositorInsertTUT6 (Tut1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
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