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Plasmid#164648PurposeTwo RGG domains tagged with mCherry and eDHFRDepositorInsertRGG
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
hsDCX-pHAT
Plasmid#83928PurposehsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorInserthuman Doublecortin isoform b (DCX Human)
UseTags6-His tag and Strep-TagIIExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A
Plasmid#127790PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2626_pGL4-UBCmin-luc2/Klf2-D'
Plasmid#124199PurposeLuciferase reporter driven by the Klf2 enhancerDepositorInsertKlf2 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(T31N)-HA
Plasmid#79405PurposeExpresses C-terminally HA-tagged Arf4(T31N) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsHAExpressionMammalianMutationT31NPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(Q71L)-HA
Plasmid#79404PurposeExpresses C-terminally HA-tagged Arf4(Q71L) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsHAExpressionMammalianMutationGln 71 LeuPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
KA1401_pGL4-UBCmin-luc2/Klf4-F'
Plasmid#124188PurposeLuciferase reporter driven by the Klf4 enhancerDepositorInsertKlf4 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1395_pGL4-UBCmin-luc2/Klf4-B'
Plasmid#124186PurposeLuciferase reporter driven by the Klf4 enhancerDepositorInsertKlf4 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A
Plasmid#32456DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationS129A, S133A, S137A (S3V, G96R, and P108L also pr…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB3042(gRNA X-4)
Plasmid#73284PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3020(gRNA X-2)
Plasmid#73282PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PtW-eSpCas9
Plasmid#133357PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsHA-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4-2 WW2
Plasmid#111883PurposeMode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: NEDD4-2 WW2 fused to C-term…ExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Atoh1wt-IRES-GFP
Plasmid#33333DepositorInsertAtoh1 (Atoh1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj10a-IRES-EGFP
Plasmid#164947PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFPDepositorInsertkcnj10a (kcnj10a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(WT)-HA
Plasmid#79403PurposeExpresses C-terminally HA-tagged Arf4(WT) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX-B3omega2 (GB1695)
Plasmid#160644PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (omega2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX-B2alpha2 (GB1696)
Plasmid#160620PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB3050(gRNA XII-5)
Plasmid#73292PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C49,50,83,103A
Plasmid#58421PurposeExpresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 49, 50, 83, and 103 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only