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Showing: 11541 - 11560 of 24268 results

  1. pEGFP-ATF6-(S1P-)

    Plasmid
    #32956
    Purpose
    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.
    Depositor
    Ron Prywes
    Article
    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-
    Insert
    ATF6 (ATF6 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S1Protease site mutation: R415A/R416A
    Promoter
    CMV
    Available since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    32956-plasmid-map-sequence-id-226826

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  4. pLM1110_LEU2

    Plasmid
    #168460
    Purpose
    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    mRFP1
    Use
    Synthetic Biology; Yeast assembly vector for big-…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168460-plasmid-map-sequence-id-365349

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  9. BNLS

    Plasmid
    #50836
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP B
    Use
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50836-plasmid-map-sequence-id-76280

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  12. MO91-dCas9-AID(P182X)

    Plasmid
    #83260
    Purpose
    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.
    Depositor
    Xing Chang
    Article
    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.
    Insert
    dCas9-AID P182X
    Use
    Retroviral
    Tags
    3xFLAG-NLS and FLAG
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9; P182X in AID
    Promoter
    MSCV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 11541 - 11560 of 24268 results