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Plasmid#50836PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).DepositorInsertddGFP B
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FSW-HRP-V5-LRRTM1
Plasmid#82536PurposeLentiviral vector for expressing HRP-LRRTM1 in neuronsDepositorInsertLRRTM1 (LRRTM1 Human)
UseLentiviralTagsHRP and V5ExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-erExRai-AKAR2
Plasmid#161756PurposeExRai-AKAR2 targeted to the cytosolic face of the ER membrane.DepositorInsertCYP450-ExRai-AKAR2
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fv-hCaspase 8 (C/A)-2A-GFP
Plasmid#82713Purposeexpress human caspase 8 in mammalian cellsDepositorInsertFv-Caspase 8 (C/A)-2A-GFP (CASP8 Human)
UseRetroviralTagsFKBP dimerization domainExpressionMammalianMutationdeleted amino acids 1-215 of caspase 8PromoterAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-mPLD4
Plasmid#201185PurposeProtein expression plasmid for recombinant His-Myc tag mouse PLD4DepositorUseTagsHis-Myc, Factor Xa cleavableExpressionMammalianMutationcytosolic and transmembrane domain truncated; add…PromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-IGK-dAPEX2-KDEL
Plasmid#117183PurposePlasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
plenti_DCLK1 G533N
Plasmid#163627PurposeExpresses DCLK1 G533N in E. coliDepositorInsertDCLK1 (DCLK1 Human)
UseLentiviralTagsV5ExpressionMutationG33NPromotersv40Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…TagsExpressionBacterialMutationPromoterPA1Available sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSP64-mHCN2
Plasmid#53060PurposeExpresses alpha subunit of mouse HCN2 channel in Xenopus oocytesDepositorInsertPotassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
UseXenopus oocyte expressionTagsExpressionMutationE55G, R237H, and K283R polymorphisms compared to …PromoterSP6Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Venus-MDGA1
Plasmid#82543PurposeOverexpression vector for Venus-MDGA1 in neuronsDepositorInsertMDGA1 (MDGA1 Human)
UseTagsVenus fluorescent proteinExpressionMammalianMutationPromoterchicken beta actinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
nuc-GafD-mTurboID-HA
Plasmid#184641PurposeExpresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome taggingDepositorInsertnuc-GlycoID-HA
UseTags3xHA tag and Nuclear localization sequenceExpressionMammalianMutationGafD -> expressed 22-178 ; BirA* -> express…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)
Plasmid#99229PurposeMammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.DepositorInsertpr-mEos2
UseTagsLifeactExpressionMammalianMutationA69T for primed conversion applicationsPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti_DCLK1 G511N
Plasmid#163626PurposeExpresses DCLK1 G511N in E. coliDepositorInsertDCLK1 (DCLK1 Human)
UseLentiviralTagsV5ExpressionMutationG511NPromotersv40Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc-GFP-dSUMO
Plasmid#85696Purposeexpression of GFP-SUMO1 in Drosophila cellsDepositorInsertSUMO (Sumo Fly)
UseTagsGFPExpressionInsectMutationPromoterAc5Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized
Plasmid#172345PurposeExpression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimizedPromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Venus-MDGA2
Plasmid#82544PurposeOverexpression vector for Venus-MDGA2 in neuronsDepositorInsertMDGA2 (Mdga2 Rat)
UseTagsVenus fluorescent proteinExpressionMammalianMutationPromoterchicken beta actinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-BtW-eSpCas9
Plasmid#133358PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsFlag-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto Grx1-roGFP2
Plasmid#64994Purposefor expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophilaDepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only