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pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

PxylA (B. megaterium)

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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p35S:Citrine-TurboID-3xHA

Plasmid

#199243

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID

Use

Tags

3xHA and TurboID-3xHA

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCH67 (crRNA expression vector)

Plasmid

#217337

Purpose

CROP-seq crRNA expression vector with GFP and puro marker

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

AsDR-filler-AsDR8, GFP-puro

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLX317 TEAD2

Plasmid

#193686

Purpose

Constitutive lentiviral expression of TEAD2

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

TEAD2 (TEAD2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K29

Plasmid

#22903

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K29 only, all other lysines mutated to arginines

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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TFORF2214

Plasmid

#142176

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREB3L4 (CREB3L4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mTurquoise2

Plasmid

#99122

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pNP566

Plasmid

#222724

Purpose

pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Recombinase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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DHB-mVenus

Plasmid

#136461

Purpose

Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

Depositor

Sabrina Spencer, Tobias Meyer

Article

Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

Insert

DNA Helicase B (HELB Human)

Use

Lentiviral

Tags

mVenus

Expression

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ132B2.0

Plasmid

#99888

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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