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Plasmid#46958PurposePlasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.DepositorInsertKu80 (XRCC5 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FRB-LgBiT
Plasmid#182650PurposeFRB-LgBiTDepositorInsertFRB-LgBiT fusion
UseTagsLgBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_HNF1A-RE_tBFP_PGK_mCitrine
Plasmid#188387PurposeLentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine;DepositorInsertHNF1A-RE_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-ERN
Plasmid#80475PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
dCas9 plasmid
Plasmid#100091PurposedCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expressionDepositorTypeEmpty backboneUseCRISPRTags3XFlag-NLS-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MAP3K5 (WT)
Plasmid#47104PurposeExpresses wild type human MAP3K5 in mammalian cellsDepositorInsertMAP3K5 (MAP3K5 Human)
UseTagsFLAGExpressionMammalianMutationNAPromoterCMVAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xERRE-luciferase
Plasmid#37851DepositorInsert3 copies of ERRE upstream of luciferase
UseLuciferaseTagsExpressionMutationPromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)
Plasmid#140005PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_9_003
Plasmid#167349PurposeddPCR gating control for droplets containing either gag+env targets OR tat + env targetsDepositorInsertgag_tat_env
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_6_002
Plasmid#167348PurposeddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targetsDepositorInsertpol_gag_tat
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only