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Plasmid#121142PurposeMammalian expression of GFP tagged CD274 with its own 3'UTRDepositorInsertCD274 with its own 3'UTR (CD274 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
1137=Hsp70Bb-Cas9[dsRed]
Plasmid#149424PurposeThe piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)DepositorInsertCas9
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro-2A-TEVp
Plasmid#99610PurposeLentivirus expressing puromycin resistance gene and TEV proteaseDepositorInsertsPuromycin resistance (PAC gene)
TEV protease
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkH
Plasmid#182291Purposeexpresses c-di-GMP biosensor in E. coliDepositorInsertmVenusNB-MrkH
UseTagsmVenusNB internal fusion to MrkHExpressionBacterialMutationPromoterproCAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTK194
Plasmid#59563PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak mKate opal attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)
Plasmid#86418PurposeONM/ER split GFP1-10DepositorInsertNOP1pr-GFP1-10-Scs2TM
UseTagsGFP1-10ExpressionYeastMutationPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK760
Plasmid#192643PurposeExpresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpRDepositorInsertBBa_J23110-sfGFP
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23110Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1201
Plasmid#44483DepositorInsertMinimal Mos1, unc-119, peel-1, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-UBE2S-UBD
Plasmid#66713PurposeExpresses the UBE2S-UBD fusion protein in E. coliDepositorUseTagsGST-3CExpressionBacterialMutationPromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp YFP-PIF-SOS2cat
Plasmid#50851PurposeOptogenetic Ras activation system; cytoplasmic componentDepositorInsertsUseLentiviralTagsYFPExpressionMutationresidues 566–1049PromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_LifeAct-iBlueberry
Plasmid#73176PurposeExpressing iBlueberry and mCherry in mammalian cellsDepositorInsertLifeAct-iBlueberry
UseTagsExpressionMammalianMutationmutation E177Q in iBlueberryPromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG:: mCherry-2a-hM4Dnrxn
Plasmid#52523PurposeCo-expresses hM4Dnrxn and a red fluorescent labelDepositorInsertmCherry-2a-hM4Dnrxn
UseTags2xHAExpressionMammalianMutationPromoterCAgAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T5-ARG1
Plasmid#106476PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917DepositorInsertARG1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
untargeted Plk1 FRET sensor Myt1 substrate
Plasmid#45217DepositorInsertuntargeted Plk1 FRET sensor Myt1 substrate
UseTagsExpressionMammalianMutationPromotert7Available sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
SBE-NLS-mCherry PGK-rtTA3
Plasmid#195332PurposeLentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3DepositorInsertsNLS-mCherry
rtTA3
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhPGK and miniCMVAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-dfROX
Plasmid#137170PurposeLentiviral transfer vector for expression of roGFP2 and HyPer RedDepositorInsertroGFP2-2A-HyPer Red
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
MmXYRS-4xM15
Plasmid#155343PurposePlasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.DepositorInsertHumanized MmXYRS/tRNAM15
UseTagsExpressionMammalianMutationPromoterCMV/U6Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6-HpaBC
Plasmid#71260PurposeExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6DepositorInsertsHpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only