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Plasmid#196711PurposeCRISPR-activation. dCas9-VP64 with TagBFP for sortingDepositorInsertdCas9-VP64-T2A-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationD10A, N863APromoterEF1aAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1_IVT
Plasmid#222342PurposePlasmid for evolved and engineered Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTemplate for in vitro transcriptionTagsExpressionMutationPromoterT7 (inactivated)Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ilux pGEX(-)
Plasmid#107879PurposeBacterial expression of ilux for bioluminescence imagingDepositorInsertilux
UseTagsExpressionBacterialMutationPromotertacAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IPP1-His
Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-D12L
Plasmid#89161PurposeExpress vaccinia virus D12L in mammalian cellsDepositorInsertVaccinia virus D12L
UseTagsExpressionMammalianMutationPromoterCAG promotorAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GST TEV LIC cloning vector (1G)
Plasmid#29655DepositorTypeEmpty backboneUseTagsHis6-GST-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
FokI-mCherry-LacI-NLS
Plasmid#215096PurposeExpresses FokI-mCherry-LacI-NLS in mammalian cells.DepositorInsertFokI-mCherry-LacI-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
hU6-pegRNA-EFS-mScarlet Template
Plasmid#196037PurposeThe template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.DepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterhU6 and EFSAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B
Plasmid#223712PurposeExpresses autophagy flux LC3B fluorescence reporterDepositorInsertLC3B (MAP1LC3B Human, Synthetic)
UseLentiviral and Synthetic BiologyTagsmCherry::EGFP::LC3BExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only