-
Plasmid#90247PurposeExpresses YFP-DAT R60A mutant in mammalian cellsDepositorInsertDAT (dopamine transporter) (SLC6A3 Human)
UseTagsExpressionMammalianMutationmutated R60 to A in human DAT cDNAPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD14
Plasmid#112822PurposepSMF2 with 14 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
UseTagsExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ctH6-eGFP
Plasmid#32562DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin siteExpressionMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101111PurposeEncodes dCas9(C)-VP64 with SAM components fused to AVPR2DepositorInsertAVPR2-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE
Plasmid#101106PurposeEncodes FKBP-dCas9(N) fused to VEGFR2DepositorInsertVEGFR2-FKBP-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pMOD_C4300
Plasmid#91103PurposeModule C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: noneDepositorInsertBaeI LacZ cassette for GT donor cloning
UseUnspecifiedTagsExpressionMutationPromoternoneAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65(150-261)-RTA(125-190)
Plasmid#99674PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-P65(151-261)-RTA(125-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-L
Plasmid#127477Purposecoding sequence for synthetic zinc finger ZF-ADD-LDepositorInsertZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
UseCell-free transcription–translationTagsPDZ cognate peptide ligand (VKESLV)ExpressionMutationPromoterT7Available sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-GCN
Plasmid#127475Purposecoding sequence for synthetic zinc finger ZF-ADD-GCNDepositorInsertZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperExpressionMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc
Plasmid#141102Purposebioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosisDepositorInsertSEP-Nanoluc
UseLentiviralTagsExpressionMutationPromoterEF1αAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P4:gs:nls:FKBP
Plasmid#135984PurposeExpresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cellsDepositorInsertP4 coiled-coil fused to the FKBP domain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB6371
Plasmid#106153PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT241_(pCA-ATG-intron-tTA2)
Plasmid#36875DepositorInserttTA2
UseTagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pconst #3-RFP
Plasmid#81077Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23101Available sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-Fast-1 H83R
Plasmid#16522DepositorInsertFAST-1 H83R (FOXH1 Human)
UseTagsExpressionMammalianMutationH83R mutantPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0039
Plasmid#117606PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)DepositorInsert[35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Sa sgRNA expression vector
Plasmid#107720PurposeFor in vitro transcription of Sa sgRNA from the T7 promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSO7
Plasmid#106365PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.DepositorInsertttTi4348_ SEC _pcol-19_OspF_3'unc54UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-PDHA1-N-10
Plasmid#56349PurposeLocalization: Mitochondria, Excitation: 530, Emission: 541DepositorInsertPDHA1 (PDHA1 Human)
UseTagsmPapayaExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only