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Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
2333_Cas9-CtIP-dnRNF168
Plasmid#200254PurposeThis plasmid expresses the Cas9-CtIP-dnRNF168 in mammalian cellsDepositorInsertCas9-CtIP-dnRNF168
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR6K6_Deletion_gRNA2
Plasmid#195196Purposedual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFPDepositorInsertOR6K6 dual gRNA (OR6K6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 CoV-2 nsp1 3xFLAG
Plasmid#176057PurposeExpressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.DepositorInsertCoV-2 nsp1 (ORF1ab SARS-CoV-2)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSTING (N154S)
Plasmid#214160PurposeTransient expression of gain-of-function STING mutantDepositorInsertSTING (N154S) (STING1 Human)
UseTagsExpressionMammalianMutationN154SPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA pCAGGS
Plasmid#206076Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain IIDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC+] (AVA2626)
Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-mCherry
Plasmid#62632Purposemammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Asp8
Plasmid#102617PurposeExpression of human RPA with aspartic acid substituted phosphorylation domainDepositorUseTagsExpressionBacterialMutationwith aspartic acid substituted phosphorylation do…PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
shYAP1 # 2
Plasmid#42541DepositorInsertYAP1 (YAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-Voltron-ST
Plasmid#119038PurposePan neuronal expression of soma-localized Voltron in zebrafishDepositorInsertVoltron-ST
UseZebrafish expressionTagsExpressionMutationPromoterelavl3Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-HaloTag-Puromycin
Plasmid#167208PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rabbit GFP Cav 2.2 pMT2
Plasmid#58737Purposeexpression of GFP tagged rabbit Cav2.2 in mammalian cellsDepositorInsertCav2.2 (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human FLAG-WDR20
Plasmid#217618PurposeMammalian expression of FLAG tagged WDR20DepositorInsertWDR20 (WDR20 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNX25-PX (506-628)
Plasmid#119104PurposeBacterial expression of human phox homology (PX) domain, SNX25-PX (506-628)DepositorInsertSNX25-PX (506-628) (SNX25 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX14-PX (561-686)
Plasmid#119095PurposeBacterial expression of human phox homology (PX) domain, SNX14-PX (561-686)DepositorInsertSNX14-PX (561-686) (SNX14 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
HT109_pAAV_hSyn-DiO-SomQuasAr6a_EGFP
Plasmid#190878PurposeCre-on expression of soma-targeted QuasAr6a under an neuronal promoterDepositorInsertsomQuasAr6a_EGFP
UseAAVTagsEGFPExpressionMutationPromoterhSynAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 P-cad
Plasmid#47502PurposeExpresses P-cadherin in mammalian cellsDepositorInsertcadherin 3, type 1, P-cadherin (placental) (CDH3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
dCas9-dMSK1
Plasmid#165602PurposeExpresses Sp dCas9 fused to truncated human MSK1 (42-802)DepositorInsertS. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 S. Pyogenes, Human, Synthetic)
UseCRISPR and LentiviralTagsFLAG TagExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PPP6C_pLX307
Plasmid#98360PurposeLentiviral expression of PPP6CDepositorInsertPPP6C (PPP6C Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only