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Plasmid#172208PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL
Plasmid#172205PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]
Plasmid#153542PurposeAAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeQ-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α1.1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]
Plasmid#153541PurposeAAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α1.1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral_394-543-V5His6_D
Plasmid#146134PurposeInsect Expression of DmTral_394-543DepositorInsertDmTral_394-543 (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations (nucleotides A198C, A387G) a…PromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E
Plasmid#146166PurposeInsect Expression of DmUPF2-siRNAresDepositorInsertDmUPF2-siRNAres (Upf2 Fly)
UseTagsExpressionInsectMutation3 non silent mutations: L1022P, V1097L and N1213S…PromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d-eGFP U6 RUNX1 g1
Plasmid#155185PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA1DepositorInsertCas13d RUNX1 gRNA1
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d-eGFP U6 RUNX1 g2
Plasmid#155186PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA2DepositorInsertCas13d RUNX1 gRNA2
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d-eGFP U6 SOX17 g1
Plasmid#155187PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA1DepositorInsertCas13d SOX17 gRNA1
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d-eGFP U6 SOX17 g2
Plasmid#155188PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2DepositorInsertCas13d SOX17 gRNA2
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-M135R-EGFP
Plasmid#164944PurposeTol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135RDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPExpressionMutationStop codon deleted; M135RPromoteractinb (actb2) promoterAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB204: UbC-GFP-Tg_SAP30L-WPRE
Plasmid#168109PurposeLentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFPDepositorInsertSAP30L (SAP30L T. guttata)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterUbCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-aACry::EGFP-zebrafish Plaat1 (WT)
Plasmid#168772PurposeExpress EGFP-zebrafish Plaat1 (WT) in zebrafish lensDepositorInsertHrasls (plaat1 Zebrafish)
UseTagsEGFPExpressionMutationPromoteralphaA-crystallin promoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C232-E05: attB2r-meGFP-attB3
Plasmid#162902PurposeGateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C
Plasmid#146001PurposeInsect Expression of DmSmaug_1-762DepositorInsertDmSmaug_1-762 (smg Fly)
UseTagsV5-6xHisExpressionInsectMutationNP_523987.1, 7 silent mutations compared to the s…PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-EGFP-T2A-RPL22-1xFlag
Plasmid#170323PurposeExpresses FLAG and EGFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP
Plasmid#168491PurposeMammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertthe E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-copGFP-Polß(WT)-puro
Plasmid#128665Purposemammalian expression of copGFP-Polß(WT) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagscopGFPExpressionMammalianMutationWild typePromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
p87AAVEGFP hp47phox (S303/304/328D)
Plasmid#112250Purposemutant p47phoxDepositorInsertp47phox (NCF1 Human)
UseAAVTagsEGFPExpressionMutationS303/304/328DPromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP
Plasmid#164945PurposeTol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFPDepositorInsertKCNJ13 (KCNJ13 Human)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationStop codon deletedPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only