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Plasmid#177831PurposeSite-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).DepositorInsertmPlum_C207G/T208C
UseTagsExpressionMammalianMutationC207G + T208CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Myc DBC1
Plasmid#35096DepositorInsertdeleted in breast cancer 1 (CCAR2 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7
Plasmid#62276Purposemammalian expression of human Alpha7 (CHRNA7)DepositorInsertCHRNA7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cas9-NAT
Plasmid#64329PurposeCas9 expression cassette carried by a yeast single-copy episome plasmidDepositorInsertHuman-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT WT (Puro)
Plasmid#154290PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationWTPromoterPGKAvailable sinceDec. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-ABK
Plasmid#126035PurposeEfficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.DepositorInsertstRNA synthetase 1
tRNA synthetase 2
tRNA for TAG codon
UseTagsExpressionBacterialMutationL274M, C313A, Y349FPromoteraraBAD promoter, glnS promoter, and proK promoterAvailable sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
YAP1-V5 in pLX304
Plasmid#42555DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2FE-ABE8e-SpRY
Plasmid#213008PurposeA lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning siteDepositorInsertABE8e-SpRY-D10A
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A nickase variant of SpRYPromoterEf-1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
YES1 Y537F
Plasmid#51299PurposeExpression of YES1 Y537FDepositorInsertYES1 (YES1 Human)
UseLentiviralTagsExpressionMutationY537FPromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4 K41M-3xFLAG
Plasmid#69838PurposeThis plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationK41MPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GACh2.0
Plasmid#121921Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0DepositorInsertGPCR activation based ACh sensor GACh2.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(WT)
Plasmid#110186PurposeExpression of mouse MeCP2 (WT) in mammalian cellsDepositorInsertMeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pECE M2-SH3PXD2A WT
Plasmid#69813PurposeExpresses M2-SH3PXD2A in mammalian cellsDepositorInsertSH3 and PX domain-containing protein 2A (SH3PXD2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40
Plasmid#100852PurposeAAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoterDepositorHas ServiceAAV1InsertjRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
PINK1 gRNA (BRDN0001147554)
Plasmid#78035Purpose3rd generation lentiviral gRNA plasmid targeting human PINK1DepositorInsertPINK1 (PINK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
T7-p53DD-pcDNA3
Plasmid#25989DepositorInserttumor protein p53 (TP53 Human)
UseTagsT7ExpressionMammalianMutationtruncated cDNA encodes truncated protein (aa 300-…PromoterAvailable sinceOct. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
STIM1-EGFP
Plasmid#210370PurposeExpresses STIM1 fused with EGFP in mammalian cellsDepositorInsertSTIM1 (STIM1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED19
Plasmid#15430DepositorInsertMED19 (Med19 Mouse)
UseTagsGST and HisExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-ArchT-GFP
Plasmid#29777PurposeAAV-mediated expression of ArchT-GFP for neuronal inhibitionDepositorInsertArchT-GFP
UseAAV; Adeno-associated virusTagsGFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV ORANGE Dlg4-HaloTag KI
Plasmid#139656PurposeEndogenous tagging of PSD95: C-terminal (amino acid position: R721)DepositorInsertgRNA and HaloTag donor
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only