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Plasmid#176066PurposeEGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassetteDepositorInsertKR
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN S473A 513A
Plasmid#175763PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513ADepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationS473A S513APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 632-639D
Plasmid#175771PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 632-639DDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutation632-639DPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shTHUMPD3-Blast
Plasmid#135690PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selectionDepositorInsertshRosa26
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shTHUMPD3-Puro
Plasmid#135691PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Puromycin selectionDepositorInsertshRosa26
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shSCR-Puro
Plasmid#135689PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selectionDepositorInsertshScrambled
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shSCR-Blast
Plasmid#135688PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selectionDepositorInsertshScrambled
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Y478C-WLS-HA-IRES-GFP
Plasmid#178063PurposeExpresssion of WLS-HA protein (Y478C mutant)DepositorInsertWLS (WLS Human)
UseTagsHA and IRES-GFPExpressionMammalianMutationY478CPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Y392C-WLS-HA-IRES-GFP
Plasmid#178062PurposeExpresssion of WLS-HA protein (Y392C mutant)DepositorInsertWLS (WLS Human)
UseTagsHA and IRES-GFPExpressionMammalianMutationY392CPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-I531T-WLS-HA-IRES-GFP
Plasmid#178064PurposeExpresssion of WLS-HA protein (I531T mutant)DepositorInsertWLS (WLS Human)
UseTagsHA and IRES-GFPExpressionMammalianMutationI531TPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-eGFP-dspB(E184Q)-6xHis
Plasmid#175783PurposePlasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and eGFPExpressionBacterialMutationE184QPromoterT7Available sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-DSep1-msfGFP
Plasmid#174494Purposebacterial expression of Drosophila Sep1 fused to monomeric superfolder GFPDepositorInsertSep1 (Sep1 Fly)
UseTagsHis6-TEV and msfGFPExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- PUb-GFPsv40 GGGG AAGA
Plasmid#174536PurposePUb-GFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsertPUb-GFP-SV40 BsaI cassette
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- PUb-GFPTub56D GGGG AAGA
Plasmid#174537PurposePUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes, with DmTub56D terminatorDepositorInsertPUb-GFP-Tub56Dterminator BsaI cassette
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR2-IR2 GFP pZa
Plasmid#170083PurposeGFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaK-IR2-IR2Available sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR: pPGK PYL1-sfGFP-HP1AI165E
Plasmid#174114PurposeExpression of PYL1-sfGFP-HP1AI165EDepositorInsertPYL1-sfGFP-HP1AI165E
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR: pPGK PYL1-sfGFP-HP1Adel72
Plasmid#174113PurposeExpression of PYL1-sfGFP-HP1Adel72DepositorInsertPYL1-sfGFP-HP1Adel72
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-myc-BioID-hp100
Plasmid#174738PurposeGene overexpressionDepositorInsertP100 (NFKB2 Human)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
Plasmid#172208PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL
Plasmid#172205PurposeAAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only