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pDB.His.MBP.3C CARD8 CARD A452C

Plasmid

#164023

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-mVenus-P2A-PuroR-HA

Plasmid

#163345

Purpose

Retroviral vector to express cytosolic mVenus and an HA-tagged puromycin selection marker

Depositor

Teunis Geijtenbeek, Louis Ates

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

mVenus

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM266 - pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159829

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159833

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Plasmid

#159838

Purpose

Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-535)

Plasmid

#135439

Purpose

Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

Plasmid

#124066

Purpose

E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-R232Q-sfGFP

Use

Tags

sfGFP

Expression

Bacterial

Mutation

R232Q

Promoter

trc

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3_altPKA-Cα-mEGFP

Plasmid

#114132

Purpose

Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

18747 (Prkaca Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMflT-o3

Plasmid

#101311

Purpose

Contains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Insert

oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)-Halo-PS

Plasmid

#86632

Purpose

Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E)-Halo-PS (AKT2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-TGAT(C242S,C253S)

Plasmid

#84336

Purpose

Expresses TGAT tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

TGAT(C242S,C253S) (TRIO Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

C242S, C253S

Promoter

CMV

Available since

Dec. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pFAB1907

Plasmid

#47857

Purpose

BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

BBa_B1006 U10

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Added two extra U at the end of Bba_1006 poly-U t…

Promoter

pLTetO12

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3989

Plasmid

#47820

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD13 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD13

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3991

Plasmid

#47822

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD15 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD15

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3993

Plasmid

#47824

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD17 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD17

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3994

Plasmid

#47825

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD18 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD18

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3995

Plasmid

#47826

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD19 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD19

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3996

Plasmid

#47827

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD20 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD20

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3998

Plasmid

#47829

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD22 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD22

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3999

Plasmid

#47830

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD23 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD23

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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