Addgene

We narrowed to 21,185 results for: crispr

Showing: 11441 - 11460 of 21185 results

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pGEM-T Easy-AtpU6-26

Plasmid

#160218

Purpose

AtpU6-26 Golden Gate level 0 piece to express gRNAs

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtU6-26 promoter

Use

Golden gate level 0 piece to express grnas

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_502_sgCD45

Plasmid

#158705

Purpose

CD45 CRISPRa control

Depositor

John Doench, David Root

Article

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD45 (PTPRC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCas9-beta

Plasmid

#169240

Purpose

Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pAGM55285

Plasmid

#153212

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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CL7-LbCas12aD156R

Plasmid

#164659

Purpose

Bacterial expression of mutated LbCas12aD156R using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a-D156R

Use

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Mutation

changed Aspartic Acid 156 to Arginine

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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M3D395A-M14-dCas9

Plasmid

#134780

Purpose

Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

METTL3/METTL14 methyltransferase domain and inactive dCas9

Use

Tags

Expression

Mammalian

Mutation

D395A

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pK7WGF2::hCas9

Plasmid

#46965

Purpose

Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

hCas9

Use

CRISPR; Plant expression

Tags

eGFP

Expression

Mutation

Promoter

35S

Available since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_hU6

Plasmid

#102840

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Use

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAB1619 hU6-BpiI-Cas13bt1-DR

Plasmid

#176314

Purpose

hU6-BpiI-Cas13bt1-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt1 crRNA direct repeat

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide B

Plasmid

#194011

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide B)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide A

Plasmid

#194008

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide A)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide B

Plasmid

#194009

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide B)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pPIK16045_pX330_Flag-SuperFi-Cas9

Plasmid

#184370

Purpose

Mammalian expression of SuperFi-Cas9 nuclease

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG and NLS

Expression

Mammalian

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

Promoter

CBh

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnxABE-flgn

Plasmid

#187932

Purpose

A-to-G base editor

Depositor

Yi Xiao

Article

et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

Insert

ecTadA(8e)-nxCas9 (D10A)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A1110

Plasmid

#91031

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

ZmUbi

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMK288 (mAID-Bsr)

Plasmid

#72826

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Bsr

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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