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Plasmid#146029PurposeInsect Expression of DmCup_419-771DepositorInsertDmCup_419-771 (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-del46-60_C
Plasmid#146043PurposeInsect Expression of DmTral-del46-60DepositorInsertDmTral-del46-60 (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug_1-583-V5His6_C
Plasmid#146000PurposeInsect Expression of DmSmaug_1-583DepositorInsertDmSmaug_1-583 (smg Fly)
UseTagsExpressionInsectMutation5 silent mutations compared to the sequence given…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug_584-762-V5His6_C
Plasmid#146002PurposeInsect Expression of DmSmaug_584-762DepositorInsertDmSmaug_584-762 (smg Fly)
UseTagsExpressionInsectMutation2 silent mutations compared to the sequence given…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug_584-999-V5His6_C
Plasmid#146003PurposeInsect Expression of DmSmaug_584-999DepositorInsertDmSmaug_584-999 (smg Fly)
UseTagsExpressionInsectMutation5 silent mutations and a deletion of the AA 961-9…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug_763-999-V5His6_C
Plasmid#146004PurposeInsect Expression of DmSmaug_763-999DepositorInsertDmSmaug_763-999 (smg Fly)
UseTagsExpressionInsectMutation2 silent mutations and a deletion of the AA 961-9…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug-A642H-V5His6_C
Plasmid#146005PurposeInsect Expression of DmSmaug-A642HDepositorInsertDmSmaug-A642H (smg Fly)
UseTagsExpressionInsectMutation10 silent mutations and a deletion of the AA 961-…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug-K606AR609A-V5His6_C
Plasmid#146007PurposeInsect Expression of DmSmaug-K606AR609ADepositorInsertDmSmaug-K606AR609A (smg Fly)
UseTagsExpressionInsectMutation10 silent mutations and a deletion of the AA 961-…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-td-sfGFP
Plasmid#180150Purposetd-sfGFP cre reporterDepositorInserttd-sfGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-RG-Linker-eGFP
Plasmid#176071PurposeEGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassetteDepositorInsertRG
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-Elo3-GFP11-Ura
Plasmid#177734PurposeER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA markerDepositorInsertElo3 (ELO3 Budding Yeast)
UseTagsGFP11 and HDELExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGal-Trans-Myc-eGFP-KDEL
Plasmid#177729PurposeER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc taggedDepositorInsertcollagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
UseTagsKDEL, Myc, and eGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP11-Ura
Plasmid#177731PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid withDepositorInsertFlucDM
UseTagsGFP11, HA, HDEL, and KAR2 Signal SequenceExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP- Ura
Plasmid#177732PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA markerDepositorInsertFlucDM
UseTagsHA, HDEL, KAR2 Signal Sequence, and eGFPExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-MTS-GFP11-Ura
Plasmid#177733PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA markerDepositorInsertMTS-GFP11
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_3
Plasmid#178049PurposeCAG tdGFP Lrig1 shRNAmir-3 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)DepositorInsertLrig1 shRNAmir
UseCre/Lox and RNAi; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2
Plasmid#178048PurposeCAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)DepositorInsertLrig1 shRNAmir
UseCre/Lox and RNAi; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1
Plasmid#178047PurposeCAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)DepositorInsertLrig1 shRNAmir
UseCre/Lox and RNAi; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-bpA-2xSV40pA-L-tdGFP-Renilla_shRNAmir
Plasmid#178046PurposeCAG tdGFP Renilla shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)DepositorInsertRenilla_713 shRNAmir
UseCre/Lox and RNAi; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir
Plasmid#178045PurposeCAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)DepositorInsertLuciferase_1309 shRNAmir
UseCre/Lox and RNAi; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only