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Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUGW-UBC-EGFP-2A-BoNT-B (LC)
Plasmid#185722PurposeLentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoterDepositorInsertbotulinum neurotoxin type B light chain
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMC037-HisMS2_PLP_pac
Plasmid#128233PurposeExpression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCKmatBC
Plasmid#138587PurposematBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, catDepositorInsertbicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
UseSynthetic BiologyTagsExpressionBacterialMutationmatB contains native GUG start codonPromoterPlac promoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-IRES-HO-1
Plasmid#80345PurposeExpresses smURFP and Heme Oxygenase-1 in mammalian cells.DepositorInsertssmURFP
HO-1
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
U6-(BbsI)sgRNA_CAG-Venus-bpA
Plasmid#86985PurposeFor cloning and expression of sgRNA together with Venus expressionDepositorInsertVenus
UseTagsExpressionMammalianMutationVenus GFP variantPromoterCAGAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_YB TATA-sfGFP-CMV_dsRed
Plasmid#117399PurposesfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-LgBiT-PhoCl1-SmBiT-MBP
Plasmid#164034Purposeluciferase-based PhoCl screening constructDepositorInsertLgBiT-PhoCl1-SmBiT-MBP
UseTagsHis tag, T7 tag, Xpress TagExpressionBacterialMutationPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-dsRed-shscramble
Plasmid#71384PurposeExpresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cellsDepositorInsertCMV promoter-dsRed-mir30-shscramble
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterCMV enhancer/promoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-4146NES(ROCK)
Plasmid#162485PurposeFor generating transgenic mice expressing ROCK biosensor based on FRET.DepositorInsertROCK biosensor based on FRET.
UseTagsYPet and ECFPExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUDP293
Plasmid#204228PurposeEpisomal yeast plasmid expressing the Ercas12a and ErCas12a gRNADepositorInsertsEubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI
UseSynthetic BiologyTagsNLSExpressionMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1 and Saccharomyces c…Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSC-5
Plasmid#62889PurposeLenti vector for expression of inducible split Cas9DepositorInsertsSpCas9(574-1368)
SpCas9(2-573)
UseCRISPR and LentiviralTagsFFBP12, FRB, NES PTK2, NLS SV40, and P2AExpressionMammalianMutation*see comment belowPromoterEFSAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFS478
Plasmid#89066PurposeKanMX Z3EV promoter cassetteDepositorInsertZ3EV promoter cassette
UseYeast integration vectorTagsExpressionMutationyeast Gal1 promoter modified with multiple Zif268…PromoterZ3EV promoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-2A-3xFLAG-SBP-2A
Plasmid#159584PurposeLuciferase reporter for ribosome pausing controlDepositorInserts2A-3xFLAG-SBP-2A
EIF2S3 5UTR
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-UGI-nCas9(H840A)-GSVG
Plasmid#187411PurposeDddA GSVG variantDepositorInsertDddA GSVG variant
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PAX6 donor plasmid
Plasmid#105239PurposeKnock in H2B-GFP into the human PAX6 locusDepositorInsertPAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
UseTALENTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK02
Plasmid#105133PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV68 L1L2
Plasmid#52587PurposeExpresses HPV68 L1 and L2 in mammalian cellsDepositorInsertsHPV68 L1
HPV68 L2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f
Plasmid#72304PurposeCombines a gCaMP-based calcium indicator and an Arch-based voltage indicatorDepositorInsertCaViar
UseLentiviralTagsTSExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only