-
Plasmid#188097PurposeExpresses human ATG13 HORMA F19D mutant in mammalian cellsDepositorInsertATG13 (ATG13 Human)
UseTagsstrep-strep-Flag tagExpressionMammalianMutationATG13 (2-197)-F19DPromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG101-Y45D
Plasmid#188105PurposeExpresses human ATG101 HORMA Y45D mutant in mammalian cellsDepositorInsertATG101 (ATG101 Human)
UseTagsGST tagExpressionMammalianMutationcodon-optimized ATG101-Y45DPromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG101-W110D/F112D
Plasmid#188106PurposeExpresses human ATG101 HORMA W110D/F112D mutant in mammalian cellsDepositorInsertATG101 (ATG101 Human)
UseTagsGST tagExpressionMammalianMutationcodon-optimized ATG101-W110D/F112DPromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-ATG13 (2-197)-L151D
Plasmid#188102PurposeExpresses human ATG13 HORMA L151D mutant in mammalian cellsDepositorInsertATG13 (ATG13 Human)
UseTagsstrep-strep-Flag tagExpressionMammalianMutationATG13 (2-197)-L151DPromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mitoX4-mVenus
Plasmid#185808PurposeMitochondrial targetted (COX8) mVenusDepositorInsertmito-mVenus
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mitoX4-FKBP-HA
Plasmid#185658PurposeMitochondrial targetted (Cox8) FKBP protein with C-terminal HA epitopeDepositorInsertmito-FKBP
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR TRF1
Plasmid#185445PurposeExpresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorInsertTRF1 (TERF1 Human)
UseTagsYBBR and ZZExpressionInsectMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKAcat(G1A)-mCherry
Plasmid#181852PurposemCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.DepositorInsertPKAcat-mCherry (PRKACA Human)
UseTagsmCherryExpressionMammalianMutationGlycine residue immediately after start site in P…PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIS_TEV_hsSYX3
Plasmid#179290PurposeE. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7lacAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-HsNot9_19-285-V181E_AE
Plasmid#148617PurposeBacterial Expression of HsNot9_19-285-V181EDepositorInsertHsNot9_19-285-V181E (CNOT9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9-shRNAres2_W
Plasmid#147905PurposeMammalian Expression of HsNot9-shRNAres2DepositorInsertHsNot9-shRNAres2 (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsNot1iso1_1356-1581_V
Plasmid#147779PurposeBacterial Expression of HsNot1iso1_1356-1581DepositorInsertHsNot1iso1_1356-1581 (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Chpt1
Plasmid#175155PurposeLentiviral expression of V5-tagged mouse Chpt1DepositorInsertChpt1 (Chpt1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClgn-Lrrc23-FLAG
Plasmid#179921PurposeVector backbone. pClgn-promoter driven expression.DepositorInsertLrrc23 (Lrrc23 Mouse)
UseTagsFLAGExpressionMammalianMutationStop codon removedPromoterCalmeginAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClgn-Lrrc23-1D4
Plasmid#179922PurposeVector backbone. pClgn-promoter driven expression.DepositorInsertLrrc23 (Lrrc23 Mouse)
UseTags1D4ExpressionMammalianMutationStop codon removedPromoterCalmeginAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Dnajc16-V5
Plasmid#175156PurposeLentiviral expression of mouse Dnajc16-V5DepositorInsertDnajc16 (Dnajc16 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Cdipt-V5
Plasmid#175144PurposeLentiviral expression of mouse Cdipt-V5DepositorInsertCdipt (Cdipt Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA Td1
Plasmid#176257PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1DepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR2
Plasmid#176245PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1
Plasmid#176250PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozymDepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…TagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only