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Plasmid#128953Purposeexpresses PiuD in E.coliDepositorInsertPutative outer membrane receptor for iron transport (PiuD)
UseTags6xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-105)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A
Plasmid#127788PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A
Plasmid#127789PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-R385A-K386A-K389A-K390A
Plasmid#127792PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in Arg3…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT3
Plasmid#118985PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationK2-mutSP-K1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT5
Plasmid#118987PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationmutK2-mutSP-K1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT7
Plasmid#118989PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationK2-mutSP-mutK1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP
Plasmid#124181PurposepiggyBac-based reporter (Puro) driven by the Pou5f1 enhancerDepositorInsertIRES-Pac, Pou5f1 enhancer
UseReporterTagsExpressionMutationPromoterUBCminAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP
Plasmid#124180PurposepiggyBac-based reporter (EGFP) driven by the Pou5f1 enhancerDepositorInsertEGFP-IRES-Pac, Pou5f1 enhancer
UseReporterTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCarCBADE
Plasmid#117757PurposeCodon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised CarCBA, Protein CarD, Ferredoxin CarE
UseTagsExpressionBacterialMutationPromoterPLac(UV5)Available sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-F219L (JBEI-7186)
Plasmid#110151PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-F219L mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PsuACR_353-EYFP
Plasmid#111076PurposeAnion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cellsDepositorInsertAnion channelrhodopsin PsuACR_353
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD2023
Plasmid#101367PurposeName: pCBR-RBPjkΔB. RBPjk binding site B mutation in pCBR-K14 (pDD2005).DepositorInsertRBPjk binding site B mutation in pCBR-K14 (pDD2005).
UseLuciferaseTagsExpressionMutationRBPjk binding site B mutation in pCBR-K14 (pDD200…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2030
Plasmid#101373PurposeName: pBluescript-RBPjkΔB. RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertRBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedTagsExpressionMutationRBPjk binding site B mutation in pBluescript-LANA…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T-1/MKRN1
Plasmid#78757PurposeTo purify GST-tagged MKRN1 recombinant proteinDepositorInsertMKRN1 (MKRN1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C103A
Plasmid#58419PurposeExpresses murine IFITM1-C103A with an N-terminal HA tag in mammalian cells.DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteine 103 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C83A
Plasmid#58418PurposeExpresses murine IFITM1-C83A with an N-terminal HA tag in mammalian cells.DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteine 83 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C50A
Plasmid#58417PurposeExpresses murine IFITM1-C50A with an N-terminal HA tag in mammalian cells.DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteine 50 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C49A
Plasmid#58416PurposeExpresses murine IFITM1-C49A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteine 49 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCru5-CCC/ACC-mEGFP-IRES-mCherry
Plasmid#49222PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only