-
Plasmid#183338PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1B
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-psba1-Ppsba2-dCas9-SpR
Plasmid#73220PurposeContains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorInsertdCas9 from S. pyogenes
UseTagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPpsbA2Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSTBlue-U6-sgRNA(tdTomato)
Plasmid#47871PurposeThis plasmid contains expression cassette for sgRNA targeting tdTomato.DepositorInsertsgRNA-tdTomato
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-N
Plasmid#113754PurposeGateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2ExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTUT5
Plasmid#60042PurposeMammalian expression of FLAG-tagged mouse TUT5DepositorInsertTUT5 (Papd7 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3012
Plasmid#91099PurposeModule C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterPvUbi1Available sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#87560PurposeExpresses aa397-423 of Flp recombinase fused to PYL CIDDepositorInsertFlpO aa397-423
UseCre/Lox and Synthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA
Plasmid#87559PurposeExpresses aa1-396 of Flp recombinase fused to ABI CIDDepositorInsertFlpO aa1-396
UseCre/Lox and Synthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_263
Plasmid#91127PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 WT
Plasmid#66139PurposeExpresses the wild type of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_NcVVD-LOV_X
Plasmid#131176PurposeExpresses NcVVD-LOV (Neurosporra crassa) followed by restriction sitesDepositorInsertNcVVD-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NmScarlet-mAID
Plasmid#140618PurposeExpression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA-ATG-intron-tdT3Myc
Plasmid#36883DepositorInserttdTomato-3Myc
UseTags3 Myc tagsExpressionMammalianMutationbeta-globin intron containing a loxP site inserte…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_AtPHYB-S_X
Plasmid#131183PurposeExpresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sitesDepositorInsertAtPHYB-S
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-7Zf-TYR
Plasmid#32786DepositorInsertTyrosinase (TYR Human)
UseIn vitro transcriptionTagsExpressionMutationI181MPromoterT7Available sinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBM23-pyrF-NT
Plasmid#174384PurposepBM23 contained pyrF and mini-CRISPR.DepositorInsertpyrF-NT
UseTemplate for cloning fragments for chromosomal in…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHFX-B60-EPSUD
Plasmid#174390PurposePlasmids for gene knockout were constructed based on suicide plasmid pHFX-B60DepositorInsertEPSUD
UseArchaeal gene knockout vector for haloferax medit…TagsExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBM23-pyrF-CS
Plasmid#174396PurposepBM23 contained pyrF and mini-CRISPR.DepositorInsertpyrF-CS
UseTemplate for cloning fragments for chromosomal in…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPI180
Plasmid#113222Purposegeneric knockin vector for C-terminal tagging with GFPDepositorTypeEmpty backboneUseGeneric knockin vector for c-terminal tagging wit…TagsGFPExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1360 - Intron GG3 - smu-1
Plasmid#159806PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG3 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only