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Plasmid#47837PurposeBIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.DepositorInsertpromoter 6 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3869
Plasmid#47842PurposeBIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.DepositorInsertpromoter 11 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRpr150-LUC
Plasmid#85700Purposeluciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)DepositorInsertRpR 150 enhancer and hsp70 minimal promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterRpR 150 enhancer and hsp70 minimal promoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(214-598)
Plasmid#135440PurposeExpresses SFPQ 214-598 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23a_mod_RBPMS_FL
Plasmid#59393PurposePlasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTags6xHis, FLAG, and HAExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Rescue Septin 11 mRNA
Plasmid#122022PurposeRescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.DepositorInsertSEPT11 (Sept11 Rat)
UseTagsExpressionMammalianMutationSilent mutations are in 5 consecutive codons (AUU…PromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lyn-CFP-GAI(1-172)
Plasmid#37313DepositorInsertGAI (GAI Mustard Weed)
UseTagsCFP and LynExpressionMammalianMutationcontains aa 1-172PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(276-535)
Plasmid#135436PurposeExpresses SFPQ 276-535 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFAB1935
Plasmid#47860PurposeBIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiencyDepositorInserthis_T_min_linker_crp_T_min double terminator
UseSynthetic BiologyTagsExpressionBacterialMutationconcatenate of his_min and crp_minPromoterpLTetO15Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJG488
Plasmid#91196PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)DepositorInsertnTaCas9_H840A+gUbi8+gUbi1+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GATA1 K137R
Plasmid#85694PurposeMammalian expression of mouse GATA1 with K137R mutationDepositorInsertGATA1 (Gata1 Mouse)
UseTagsExpressionMammalianMutationK137R (SUMO-1 attachment site)PromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP
Plasmid#127363PurposeBinary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
UseTagsGS linker, NES, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lyn-CFP-GAI(1-151)
Plasmid#37312DepositorInsertGAI (GAI Mustard Weed)
UseTagsCFP and LynExpressionMammalianMutationcontains aa 1-151PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 cyto Grx1-roGFP2
Plasmid#64999PurposeExpression of cytosolic Grx1-roGFP2 under the alphaTub84B promoterDepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lyn-CFP-GAI(1-532)
Plasmid#37314DepositorInsertGAI (GAI Mustard Weed)
UseTagsCFP and LynExpressionMammalianMutationcontains aa 1-532PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE-PDS3_IMS
Plasmid#112875Purposebinary vector for IMS (Induced Mis-Splicing) of PDS3 in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)
Plasmid#159863PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-CT
Plasmid#64063Purposemyc-tagged murine seipin (cytosolic C-terminus); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationContains Arg326 to Ser443PromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT9753-BEAR-mScarlet-preedited
Plasmid#162994PurposeBEAR control plasmid with split mScarlet and intact 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-mTurquoise2-P2A-PuroR-HA
Plasmid#163344PurposeRetroviral vector to express cytosolic mTurquoise2 and an HA-tagged puromycin selection markerDepositorInsertmTurquoise2
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only