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CDK17 gRNA (BRDN0001149533)

Plasmid

#76785

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK17

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK17 (CDK17 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK17 gRNA (BRDN0001149453)

Plasmid

#76786

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK17

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK17 (CDK17 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CDK17 gRNA (BRDN0001147014)

Plasmid

#76787

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK17

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK17 (CDK17 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CDK17 gRNA (BRDN0001146845)

Plasmid

#76788

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK17

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK17 (CDK17 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PFKFB4 gRNA (BRDN0001149495)

Plasmid

#77123

Purpose

3rd generation lentiviral gRNA plasmid targeting human PFKFB4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PFKFB4 (PFKFB4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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PFKFB4 gRNA (BRDN0001146962)

Plasmid

#77124

Purpose

3rd generation lentiviral gRNA plasmid targeting human PFKFB4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PFKFB4 (PFKFB4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
STYK1 gRNA (BRDN0001145785)

Plasmid

#75695

Purpose

3rd generation lentiviral gRNA plasmid targeting human STYK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STYK1 (STYK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STYK1 gRNA (BRDN0001146810)

Plasmid

#75696

Purpose

3rd generation lentiviral gRNA plasmid targeting human STYK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STYK1 (STYK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-NtermRGS2-GFP-CIBN

Plasmid

#112258

Purpose

Expresses alpha-helix component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

NtermRGS2-GFP-CIBN

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1322

Plasmid

#111109

Purpose

Expresses FANCL_+_58468435.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCL gRNA (FANCL Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (E563K)

Plasmid

#29541

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pLV UbC6-ARRET

Plasmid

#111378

Purpose

ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Insert

ARRET polyA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UbC6

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1271

Plasmid

#111106

Purpose

Expresses FANCI_-_89787239.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCI gRNA (FANCI Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-235

Plasmid

#90509

Purpose

pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - Hy2, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and phytochromobilin:ferredoxin oxidoreductase (Hy2)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-sHO1, mito-Hy2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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MELK E6.2 gRNA

Plasmid

#90767

Purpose

3rd generation lentiviral gRNA plasmid targeting human MELK

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MELK (Guide Designation E6.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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FH95pUP95W (E6)

Plasmid

#74036

Purpose

lentiviral expression of Psd95 shRNA and Psd95 (no GFP)

Depositor

Oliver Schluter, Weifeng Xu, Robert Malenka

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
FgH1tUTG_huMcl-1.2

Plasmid

#85531

Purpose

Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Mcl-1.2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K12 gRNA (BRDN0001144753)

Plasmid

#76760

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K12

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K12 (MAP3K12 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAP3K12 gRNA (BRDN0001147643)

Plasmid

#76761

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K12

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K12 (MAP3K12 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge