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pTH731-2µ-RLuc/minCFLuc
Plasmid#40601DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W
Plasmid#67980PurposeCas9 activity reporter with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWZL-Neo-Myr-Flag-DEST
Plasmid#15300Purposea Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORFDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-WT-WLS-HA-IRES-GFP
Plasmid#178061PurposeExpresssion of WLS-HA protein (WT)DepositorInsertWLS (WLS Human)
UseTagsHA and IRES-GFPExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV2 Nucleocapsid
Plasmid#153201PurposeExpresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cellsDepositorInsertNucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
UseTagsMyc tag (5X)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBabe-Neo-Flag-IKBKE
Plasmid#15265DepositorInsertIKBKE (IKBKE Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-sgCD20
Plasmid#209749PurposeLentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-cRAF-W22
Plasmid#15270DepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationC-terminal deletionPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V3-CD20-Puro
Plasmid#209757PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11
Plasmid#161923PurposeTo generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.DepositorInsertsgRNA targeting HSD17B11 exon 1
UseCRISPRTagsExpressionMutationPromoterU6Available sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL 4K>R
Plasmid#202420PurposeExpression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)DepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationK431/525/526/547RPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Neo-Myr-Flag-IKBKE
Plasmid#15267DepositorInsertIKBKE (IKBKE Human)
UseRetroviralTagsMyr-FlagExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-EGFP MASTL G44S (siRNA resistant)
Plasmid#191012PurposeExpresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)DepositorInsertMicrotubule-associated serine/threonine kinase like (MASTL Human)
UseTagsEGFPExpressionMammalianMutationG44SPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta EC
Plasmid#202423PurposeExpression of GFP-tagged PODXL without extracellular domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationdeleted extracellular domainPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL 5N>Q
Plasmid#202419PurposeExpression of GFP-tagged PODXL with mutated N-linked glycosylation sitesDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationN33/43/104/144/360QPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTH734-2µ-RLuc/stopCFLuc
Plasmid#40609DepositorInsertsFirefly Luciferase (nonsense mutant)
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCSC-LHX8-IRES-GBX1
Plasmid#182235PurposeTo convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189DepositorUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only
