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Showing: 1121 - 1140 of 7971 results
  1. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid
    #70659
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4

    Plasmid
    #62128
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid
    #133340
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus
    Depositor
    Insert
    sgRNA_gcrA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid
    #133793
    Purpose
    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017
    Insert
    CjeCas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    V2-TGAA linker sgRNA architecture from Kim et al.…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid
    #124541
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Insert
    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT, WT
    Promoter
    Minimal ADE
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5

    Plasmid
    #62127
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid
    #124539
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Insert
    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    d11-41 C55A, d11-41
    Promoter
    Minimal ADE
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pMuLE ENTR U6 stuffer sgRNA scaffold L5-L4

    Plasmid
    #62129
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1121 - 1140 of 7971 results