Addgene

We narrowed to 1,400 results for: pbluescript

Showing: 1121 - 1140 of 1400 results

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pGWKS12

Plasmid

#139419

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Superfolder GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Nanog Hyg

Plasmid

#13836

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

IRES-Hyg-pA cassette flanked by Nanog homologous regions (Nanog Mouse)

Use

Beta-gal

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
2674 pBS Bcl-2 P1+P2

Plasmid

#8746

Depositor

Stanley Korsmeyer

Article

Young et al Mol Cell Biol 1993 Jun;13(6):3686-97.

Insert

Bcl-2 P1+P2 (BCL2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pGFPmAID_htz-1

Plasmid

#174214

Purpose

Donor plasmid for tagging htz-1 with GFP::mAID

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

GFP::mAID::htz-1 tagging donor

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Trpc2-IRES-taumCherry-ACNF TV

Plasmid

#105212

Purpose

targeting vector to generate a Trpc2-IRES-taumCherry mouse strain.

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Trpc2-IRES-taumCherry-ACNF (Trpc2 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only
pCA-T-intron-G

Plasmid

#36887

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron-T

Plasmid

#36885

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-Squ5'3'

Plasmid

#20165

Depositor

Eric Wieschaus

Article

Martin et al Nature. 2008 Nov 23. ():.

Insert

spaghetti squash (squ, myosin II RLC) 5' UTR, ORF (without stop codon) and 3' UTR with multiple cloning site downstream (sqh Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pCA-ATG-intron-tdT3Myc

Plasmid

#36883

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tdTomato-3Myc

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

beta-globin intron containing a loxP site inserte…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-IT10

Plasmid

#66871

Purpose

Inntag 10: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 10

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only
SauCas9KKH CCR5-nicking sgRNA

Plasmid

#169863

Purpose

SauCas9KKH nicking sgRNA for CCR5

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9KKH CCR5-nicking sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5

Plasmid

#52537

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pISH-Lhx2

Plasmid

#74337

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Lhx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

LIM homeobox protein 2 (Lhx2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5

Plasmid

#52538

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pGWKS2

Plasmid

#139410

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

GFP S65T

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBS ColI alpha2

Plasmid

#19207

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 type I collagen (COL1A2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS ALS2

Plasmid

#19228

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Amyotrophic lateral sclerosis 2 (juvenile) (ALS2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
3087 pBS Bcl-2-Ig

Plasmid

#8809

Depositor

Stanley Korsmeyer

Article

Seto et al EMBO J 1988 Jan;7(1):123-31.

Insert

Bcl-2-Ig (BCL2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
psbA2-Ptrc-T7-g10-PHLS (e)

Plasmid

#52310

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc-T7

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVA009

Plasmid

#22842

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA containing CHLI fragment

Use

Cotton vigs vector

Tags

Expression

Bacterial

Mutation

Cotton Leaf Crumple Virus Coat Protein (AR1 gene)…

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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