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297_pETcon_SARS2_Omicron-BA286
Plasmid#222231Purposeyeast surface display of the SARS-CoV-2 BA.2.86 variant RBDDepositorInsertSARS-CoV-2 BA.2.86 RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Butelase
Plasmid#221848PurposeExpresses asparaginyl endopeptidase butelaseDepositorInsertButelase 1 ligase
UseTagsExpressionBacterialMutationNoPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ub-LPNTG-pET
Plasmid#221850PurposeExpresses Ub with sortase motif and His tagDepositorInsertUbiquitin
UseTagsExpressionBacterialMutationArg74ProLeuThrPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaM
Plasmid#224428PurposeExpress AzaM in E. coliDepositorInsertazaM
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaC
Plasmid#224424PurposeExpress AzaC in E. coliDepositorInsertazaC
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaD
Plasmid#224425PurposeExpress AzaD in E. coliDepositorInsertazaD
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaB
Plasmid#224423PurposeExpress AzaB in E. coliDepositorInsertazaB
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaG
Plasmid#224427PurposeExpress AzaG in E. coliDepositorInsertazaG
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaE
Plasmid#224426PurposeExpress AzaE in E. coliDepositorInsertazaE
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET11a-AldoA
Plasmid#224925PurposeInducible expression of human aldolase ADepositorInsertAldolase A (ALDOA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1(4H)-2T_pET47b
Plasmid#224069PurposeBacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage siteDepositorInserttlnrd1 (TLNRD1 Human)
UseTags6xHISExpressionBacterialMutationL191T/A225T double mutant; reduces affinity of TL…PromoterT7Available sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
TLNRD1(4H)-2E_pET151
Plasmid#224068PurposeBacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage siteDepositorInserttlnrd1 (TLNRD1 Human)
UseTags6xHIS; V5ExpressionBacterialMutationK192E/R233E double mutant; prevents actin binding…PromoterT7Available sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
CCM2(FL)_pET49b
Plasmid#224066PurposeBacterial expression of full-length CCM2 fused to an N-terminal GST. HRV-3C cleavage siteDepositorInsertccm2 (CCM2 Human)
UseTagsGST; 6xHISExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET20b-mPTER
Plasmid#222189PurposeBacterial expression of mouse PTER proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-DDX53
Plasmid#222205PurposeExpress DDX53 protein in bacteriaDepositorInsertDDX53 (DDX53 Human)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-C-MBP
Plasmid#214304PurposeProvide a bacterial expression vector that encodes C-terminus MBP tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pet28a+_Yjjg
Plasmid#220228PurposeBacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similarDepositorInsertPyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
UseTagsHistagExpressionBacterialMutationPromoterT7Available sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only