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Plasmid#190377PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::BetI-VP16_19St)DepositorInsertproG1090::BetI-VP16_19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2494
Plasmid#190376PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-VP16_19St)DepositorInsertproG1090::RpaR-VP16_19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2281
Plasmid#190362PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-ERF2(AD)-19St)DepositorInsertproG1090::AmtR-ERF2(AD)-19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2180
Plasmid#190353PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::TetR-VP16-19St)DepositorInsertproG1090::TetR-VP16-19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2156
Plasmid#190345PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-VP16-19St)DepositorInsertproG1090::AmtR-VP16-19St
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I10 PhiC31 GG2-GG4
Plasmid#195893PurposeLevel 0 PhiC31 integrase with GG2 GG4 golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I11 PhiC31-NLS GG1-GG4
Plasmid#195894PurposeLevel 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I12 PhiC31-NLS GG2-GG4
Plasmid#195895PurposeLevel 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I14 DST GG4-GG5
Plasmid#195896PurposeLevel 0 DST (destabilization RNA tag: SAUR tag) with GG4 GG5 golden gate linkersDepositorInsertDST
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_full-site
Plasmid#186277PurposeReporter plasmid for full-site selection assayDepositorInsertThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#186276PurposePlasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assaysDepositorInsertThiomicrospira shortened CRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH009_proD_Cas12c-sgRNA-GFP_CAM
Plasmid#183070PurposePlasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertCas12c single guide RNA targeting GFP
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSin4 UAP1 F383G
Plasmid#169891PurposeUsed for generation of stable mammalian cell lines expressing UAP1 with with F383G mutationDepositorInsertUDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)
UseLentiviralTagsNoneExpressionMammalianMutationChanged Phenylalanine (F) at position 383 to glyc…PromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_083 Cas12i1
Plasmid#171670PurposeFor bacterial expression of Cas12i1 with an N-terminal His-MBP tagDepositorInsertCas12i1
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1141
Plasmid#160867PurposePJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15ADepositorInsertConiferyl alcohol acyltransferase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL:P3S + STS:P4S
Plasmid#139789Purposeexpression of biosynthetic enzymes 4CL and STS in fusion with CCDepositorInsertbiosynthetic enzymes 4CL and STS in fusion with coiled-coil segments
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW218
Plasmid#113240PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flipDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationAAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…PromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD-MTS2 (12)
Plasmid#101698PurposeMTS2 in domestication vectorDepositorInsertCCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA7
Plasmid#83871PurposeGateway donor vector for IAA7DepositorInsertIAA7 (IAA7 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA8
Plasmid#83873PurposeGateway donor vector for IAA8DepositorInsertIAA8 (IAA8 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2016AvailabilityAcademic Institutions and Nonprofits only