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Addgene
Showing: 1121 - 1140 of 1781 results

  1. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191163
    Purpose
    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587:EGFP-3xMS2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Availability
    Academic Institutions and Nonprofits only
    191163-plasmid-map-sequence-id-375409

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  3. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190034
    Purpose
    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A134V,A140V,A141V,A143V
    Promoter
    CBh, SV40, hU6
    Availability
    Academic Institutions and Nonprofits only
    190034-plasmid-map-sequence-id-371641

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  4. pNB22

    Plasmid
    #103059
    Purpose
    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp
    Depositor
    Dirk Bumann
    Article
    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-
    Insert
    GFP-OVA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Promoter
    hmpAp
    Availability
    Academic Institutions and Nonprofits only
    103059-plasmid-map-sequence-id-199053

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  5. pNB23

    Plasmid
    #103058
    Purpose
    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp
    Depositor
    Dirk Bumann
    Article
    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-
    Insert
    GFP-OVA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hydrogen peroxide-inducible promoter katGp
    Availability
    Academic Institutions and Nonprofits only
    103058-plasmid-map-sequence-id-199054

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  8. Linked mRuby3 VLP

    Plasmid
    #182430
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Li Chen Cheah et al BioRxiv, 2022
    Inserts
    VP2C-GFP-mRuby3
    deltaVP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Availability
    Academic Institutions and Nonprofits only
    182430-plasmid-map-sequence-id-360112

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  9. pHL 1023

    Plasmid
    #53013
    Purpose
    PLtetO-1:mCherry, PLlacO-1:gfp (st7)
    Depositor
    Han Lim
    Article
    Hussein et al Nucleic Acids Res. 2012 May 22.
    Inserts
    mCherry
    gfp (st7)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Availability
    Academic Institutions and Nonprofits only

  10. pHL 342

    Plasmid
    #53012
    Purpose
    PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Nucleic Acids Res. 2012 May 22.
    Inserts
    mCherry
    araC
    ompC
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains -81 to +36 relative to start codon
    Promoter
    Plar, pLlacO-1, and pLtetO-1
    Availability
    Academic Institutions and Nonprofits only

  15. p3991

    Plasmid
    #198149
    Purpose
    Mammalian expression of LAMP1
    Depositor
    Michael Hensel
    Insert
    PCMV::LAMP1::HaloTag::GFP (LAMP1 Human)
    Use
    Tags
    HaloTag-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    198149-plasmid-map-sequence-id-388089

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  17. pTALE64G

    Plasmid
    #35188
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition
    Depositor
    Gang Bao
    Insert
    TAL Effector-VP64-T2A-eGFP
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain, T2A-GFP
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    35188-plasmid-map-sequence-id-42839

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  18. Coexpressed mRuby3 VLP

    Plasmid
    #182431
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Li Chen Cheah et al BioRxiv, 2022
    Inserts
    VP2C-GFP
    deltaVP1
    VP2C-mRuby3
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Availability
    Academic Institutions and Nonprofits only
    182431-plasmid-map-sequence-id-360113

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Showing: 1121 - 1140 of 1781 results