Skip to main content
Addgene
Showing: 1121 - 1140 of 1179 results
  1. AKARdual

    Plasmid
    #99869
    Purpose
    Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate
    Depositor
    Insert
    AKAR4
    Use
    Tags
    LSSmOrange and mKate2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pLPT234

    Plasmid
    #127855
    Purpose
    Repressilator 2.0, without degradation tags, integrated triple reporter
    Depositor
    Inserts
    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    First 11aa derived from mCherry as fusion protein
    Promoter
    pAmpR, pL LacO1, pL tetO1, and pR
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pDC-UAS-nBitbow1.0(miniW)

    Plasmid
    #138329
    Purpose
    Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.
    Depositor
    Inserts
    mAmetrine
    tdKatushka2
    mNeonGreen
    mTFP1
    mKusabira-Orange2
    Use
    Tags
    human H2B
    Expression
    Insect
    Mutation
    Promoter
    10xUAS
    Available since
    March 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid
    #207148
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Insert
    GNAI1 (GNAI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)K
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1(+)Gα15-244-NLuc

    Plasmid
    #207154
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Insert
    GNA15 (GNA15 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position E(244)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

    Plasmid
    #105210
    Purpose
    Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)
    Depositor
    Insert
    SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))

    Plasmid
    #105207
    Purpose
    Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)
    Depositor
    Insert
    SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

    Plasmid
    #105209
    Purpose
    Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)
    Depositor
    Insert
    SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1121 - 1140 of 1179 results