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Plasmid#192271PurposeDonor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporterDepositorInsertPromoter Reporter Insert within donor template
UseCRISPRTagsExpressionMutation-124 C to TPromoterTERT promoter driving expression of gaussiaAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TERTp146_Gluc_CCR5
Plasmid#192272PurposeDonor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporterDepositorInsertPromoter Reporter Insert within donor template
UseCRISPRTagsExpressionMutation-146 C to TPromoterTERT promoter driving expression of gaussiaAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-63-SFFV-T2A-BlaR-polyA-R11a
Plasmid#179894PurposeDonor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)DepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsExpressionBacterial and MammalianMutationPromoterSFFVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker(A6TAG)-3xFLAG KI
Plasmid#182680PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.DepositorInsertNefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-61-SFFV-T2A-PuroR-polyA-R11a
Plasmid#179892PurposeDonor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock outDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsExpressionBacterial and MammalianMutationPromoterSFFVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-62-SFFV-T2A-PuroR-polyA-R11b
Plasmid#179893PurposeDonor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)DepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsExpressionBacterial and MammalianMutationPromoterSFFVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-64-SFFV-T2A-BlaR-polyA-R11b
Plasmid#179895PurposeDonor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)DepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsExpressionBacterial and MammalianMutationPromoterSFFVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FluoSTEP-AKAR (C/Y)
Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
UseTagsCFP(1-10) and cpVenus(E127)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P50L
Plasmid#60533PurposeDonor vector for human Pak5 P50L mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationP50LPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P76T
Plasmid#60534PurposeDonor vector for human Pak5 P76T mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationP76TPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 S312C
Plasmid#60535PurposeDonor vector for human Pak5 S312C mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationS312CPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V463L
Plasmid#60536PurposeDonor vector for human Pak5 V463L mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationV463LPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V593I
Plasmid#60539PurposeDonor vector for human Pak5 V593I mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationV593IPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
GK Rab3A T36N p201
Plasmid#44697DepositorInsertRab3A (RAB3A Human)
UseGateway donor vectorTagsExpressionMutationT37NPromoterAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
GK Rab27A p201
Plasmid#44705DepositorInsertRAB27A (RAB27A Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
GK Rab3A Q81L p201
Plasmid#44698DepositorInsertRab3A (RAB3A Human)
UseGateway donor vectorTagsExpressionMutationQ81LPromoterAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
GK Rab6A’ T27N p201
Plasmid#44702DepositorInsertRab6A' (RAB6A Human)
UseGateway donor vectorTagsExpressionMutationT27NPromoterAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR SC4MOL 519
Plasmid#27981DepositorInsertSC4MOL (MSMO1 Human)
UseGateway donor vectorTagsExpressionMutationH173Q mutation in SC4MOLPromoterAvailable sinceMarch 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb
Plasmid#183078PurposerAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.DepositorInsertBRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
UseAAV; Homologous recombination donor templateTagsExpressionMutationV600E (T>A), S607S (TCC>AGT)PromoterNone for primary insert. PGK for puromycin resist…Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-DsRed
Plasmid#64703PurposeVector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsertpiggyBac-3xP3-DsRed
UseTagsExpressionMutationPromoter3xP3Available sinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only