Showing: 1121 - 1140 of 4601 results
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Plasmid#70083PurposeBacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression.DepositorInsertDnd1 (DND1 Human)
UseTags6xHIS and FLAGExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-GFP(1-10)opti
Plasmid#129416PurposeExpressing codon-optimized GFP(1-10) fragment in human cellsDepositorInsertcodon-optimized GFP(1-10)
UseCRISPR, TALEN, and Unspecified ; Human safe harbo…TagsExpressionMammalianMutationPromoterEF-1αAvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q03JI6
Plasmid#103123PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ03JI6 (Cas9 coding gene from Campylobacter jejuni)
UseCRISPR; Cloning vectorTagsSV40NLSExpressionMutationhuman codon-optimizedPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTE3330
Plasmid#107534PurposeExpresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.DepositorInsertsLb crRNA
hLbCpf1(RVR mutant)
UseTags3xHA and NLSExpressionMammalianMutationG532R, K538V, Y542RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3327
Plasmid#107535PurposeExpresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.DepositorInsertsAs crRNA
hAsCpf1(RVR mutant)
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationS542R, K548V, N552RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3331
Plasmid#107536PurposeExpresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.DepositorInsertsFn crRNA
hFnCpf1(RVR mutant)
UseTags3xHA and NLSExpressionMammalianMutationN607R, K613V, N617RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3334
Plasmid#107537PurposeExpresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.DepositorInsertsMb crRNA
hMbCpf1(RVR mutant)
UseTags3xHA and NLSExpressionMammalianMutationN576R, K582V, N586RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3329
Plasmid#107538PurposeExpresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.DepositorInsertsLb crRNA
hLbCpf1(RR mutant)
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationG532R, K595RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3328
Plasmid#107539PurposeExpresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.DepositorInsertsAs crRNA
hAsCpf1(RR mutant)
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationS542R, K607RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3333
Plasmid#107540PurposeExpresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.DepositorInsertsFn crRNA
hFnCpf1(RR mutant)
UseTags3xHA and NLSExpressionMammalianMutationN607R, K671RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3336
Plasmid#107541PurposeExpresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.DepositorInsertsMb crRNA
hMbCpf1(RR mutant)
UseTags3xHA and NLSExpressionMammalianMutationN576R, K637RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt
Plasmid#157701Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt
Plasmid#157702Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nprot-HF_GC3opt
Plasmid#157732Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoterDepositorInsertNprot-HF_GC3opt (N SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pBOB-CAG-SARS-CoV2-Spike-HA
Plasmid#141347Purpose3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorHas ServiceLentiviral PrepInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAExpressionMutationresynthesized with human codon optimization nucle…PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)
Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid#140003PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)
Plasmid#114089PurposeCAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized FnCas12a
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1121 - 1140 of 4601 results