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NSP3C-mCherry

Plasmid

#210377

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3C (ORF1ab SARS-CoV-2)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pαH-rS2d

Plasmid

#183516

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein S-GSAS-rS2d with (682-685) furin side replaced with GSAS and mutation S383C, D985C

Depositor

Priyamvada Acharya

Article

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

rS2d (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2G2A

Plasmid

#174334

Purpose

encodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

mCherry tag

Expression

Mammalian

Mutation

mutation of a.a 2 Gly to Ala

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

Plasmid

#185451

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-nCoV2019-N-YBBR-2xStrep-IRES-Puro

Plasmid

#185450

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal YBBR and Strep tag in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

Strep-Tag II and YBBR

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Cdx2

Plasmid

#70697

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Cdx2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Cdx2 (Cdx2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-2P-OMICRON

Plasmid

#180593

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Article

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Promoter

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2

Plasmid

#18047

Depositor

Edward Yeh

Article

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2m

Plasmid

#18713

Depositor

Edward Yeh

Article

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R577L, K578M

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pGC682

Plasmid

#107020

Purpose

GFP reporter of lag-2 driven by 1 kb of promoter, which lackes DBS motif

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

lag-2

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_SEPT2

Plasmid

#71547

Purpose

mammalian expression of Sept 2 with a GFP fusion

Depositor

Elias Spiliotis

Article

Bowen et al J Cell Biol. 2011 Jul 25;194(2):187-97.

Insert

Septin 2 (Septin2 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGAD424-hSyntenin2b

Plasmid

#89427

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Use

Tags

Gal4 AD

Expression

Yeast

Mutation

*see below

Promoter

ADH1

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-hSyntenin2a

Plasmid

#89436

Purpose

mammalian expression of EGFP tagged human Syntenin2a

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBT9-hSyntenin2a

Plasmid

#89421

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Use

Tags

GAL4 DBD

Expression

Yeast

Mutation

Promoter

ADH1

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGBT9-hSyntenin2b

Plasmid

#89422

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Use

Tags

GAL4 DBD

Expression

Yeast

Mutation

* see below

Promoter

ADH1

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC684

Plasmid

#107022

Purpose

GFP reporter of lag-2 driven by 1 kb of promoter, which lackes both DBS and Cons motifs

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

lag-2

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD424-hSyntenin2a

Plasmid

#89426

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Use

Tags

Gal4 AD

Expression

Yeast

Mutation

Promoter

ADH1

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmpr2ΔEC gRNA resistant

Plasmid

#163413

Purpose

Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The extracellular domain (27-150 aa) is deleted. …

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-hSyntenin2b

Plasmid

#89437

Purpose

mammalian expression of EGFP tagged human Syntenin2b

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

*see below

Promoter

CMV

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00173

Plasmid

#34326

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7807 (AP-2 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only