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Plasmid#69448Purposegenomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead AubDepositorInsertaubergine (aub Fly)
UseTagsNAExpressionInsectMutationchr2L:10,998,626(−)T>GPromoteraubergineAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASt- Crb intraGFP
Plasmid#67525PurposeExpression of transmembrane and the intracellular domains of Crb with GFP.DepositorInsertcrumbs (crb Fly)
UseDrosophilaTagsGFPExpressionInsectMutationaa97-2103 deleted (extracellular domain)Promoterhsp70Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
CJ20 (miR-1003 minigene)
Plasmid#17761DepositorInsertdme-miR-1003 minigene
UseDrosophila metallothionein gene promoter drives e…TagsExpressionInsectMutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
FoRC.PGm
Plasmid#84586PurposeDrosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
CJ31 (miR-1006 minigene)
Plasmid#17768DepositorInsertdme-miR-1006 minigene
UseDrosophila p-element vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
Renilla luciferase-Pol III
Plasmid#37380DepositorInsertPolIII-Renilla control reporter (RpIII128 Fly)
UseLuciferaseTagsExpressionMammalianMutationPromoterRNA PolIII 128 subunit (RpIII128)Available sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pB[nos-Cas9 PUb-ZsGreen]
Plasmid#169011PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster nanosAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-Hsp70-Cas9
Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::DsRed, Pactin::Ptrsps}
Plasmid#86862PurposeExpresses DsRed in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
UseTagsExpressionInsectMutationS129APromoterAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB[vasa-Cas9 PUb-ZsGreen]
Plasmid#169012PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster vasaAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::EGFP, Pactin::Ptrsps}
Plasmid#86861PurposeExpresses EGFP in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D CA
Plasmid#52247PurposeDrosophila expression of YFP-Ras85D constitutively active mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ61L; CA mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D WT
Plasmid#52242PurposeDrosophila expression of YFP-Ras85DDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D DN
Plasmid#52246PurposeDrosophila expression of YFP-Ras85D dominant negative mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS17N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGC(2.1)-U6.3
Plasmid#170516PurposePCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFPDepositorInsertgRNAcore(2.1)-pU6.3
UsePcr template vector for amplifying grnacore(2.1)-…TagsExpressionMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pT7-MS2-HA-C1-MBP-DmBam_13-36_AG
Plasmid#148839PurposeMammalian Expression of DmBam_13-36DepositorInsertDmBam_13-36 (bam Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-2_dCrk
Plasmid#131146PurposeExpresses GST::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon
Plasmid#69899PurposeExpresses the human HIPK3 exon 2 circular RNA in mammalian cellsDepositorInsertHIPK3 (stw Fly, Human)
UseTagsExpressionMammalianMutationExpresses aa1-194 of HIPK3PromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only