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We narrowed to 2,422 results for: BFP

Showing: 1121 - 1140 of 2422 results

Results per page:

pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Availability

Academic Institutions and Nonprofits only

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L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

Plasmid

#219690

Purpose

Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

Depositor

Jennifer Nemhauser

Insert

T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)

Use

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Mutation

Promoter

CBh and U6

Availability

Academic Institutions and Nonprofits only

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pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64219

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Mutation

Promoter

CBh and U6

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

Plasmid

#64220

Purpose

Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

Expression

Mammalian

Mutation

Promoter

CBh and U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

Plasmid

#78251

Purpose

Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-loxP-mTagBFP2-stop-
mCherry-NS-MCS

Use

Zebrafish motor neuron inducible expression

Tags

Expression

Mutation

Promoter

-3mnx1

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

Plasmid

#60910

Purpose

dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, and NLS

Expression

Mammalian

Mutation

Promoter

dSV40 Promoter

Availability

Academic Institutions and Nonprofits only

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MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

Plasmid

#128607

Purpose

Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Availability

Academic Institutions and Nonprofits only

Enlarge
SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

Plasmid

#128608

Purpose

Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1108 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆RRM]

Plasmid

#107840

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of RRM domains in splicing reporter

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1109 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆CTD]

Plasmid

#107841

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of C-terminal domain in splicing reporter

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1138 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆NTD]

Plasmid

#107842

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of N-terminal domain in splicing reporter

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]

Plasmid

#107844

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All hydrophobics combined in two clusters in spli…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]

Plasmid

#107845

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All hydrophobics combined in four clusters in spl…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xΦ]

Plasmid

#107846

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All hydrophobics combined in six clusters in spli…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1335 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295S]

Plasmid

#107848

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G295S in splicing reporter

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1337 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309S]

Plasmid

#107849

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G309S in splicing reporter

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1339 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348S]

Plasmid

#107850

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G348S in splicing reporter

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1285 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309F]

Plasmid

#107851

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G309F in splicing reporter

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1338 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348V]

Plasmid

#107852

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G348V in splicing reporter

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge