Addgene

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pFETCh_RXRB

Plasmid

#104040

Purpose

Donor vector for 3' FLAG tag of human RXRB

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RXRB homology arms (RXRB Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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MIS12-FRB-FLAG

Plasmid

#114040

Purpose

Kinetochore localised FRB tagged with FLAG

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

MIS12 (MIS12 Human)

Use

Tags

FRB-FLAG-tagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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OA-1067L

Plasmid

#200253

Purpose

pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

6 gRNAs targeting Doublesex, Intersex, bTublin

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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RVD-TG1

Plasmid

#173404

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NG-NN dimer RVD at Position1

Use

Unspecified

Tags

Expression

Mutation

no

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4_HA-Halo-cmyc-GRM3 C127A

Plasmid

#140458

Purpose

Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A

Depositor

Erik Procko

Insert

GRM3 (GRM3 Human)

Use

Tags

Halo, Signal/leader sequence from influenza hemag…

Expression

Mammalian

Mutation

Cysteine 127 to alanine, Threonine 174 to alanine

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTL343

Plasmid

#168404

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OsaHIP09

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585-gp130

Plasmid

#174404

Purpose

Bacterial expression of sarA-gp130 chimera under native promoter.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA-IL6ST chimera

Use

Tags

Expression

Bacterial

Mutation

Promoter

Endogenous

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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PW124-LMNA 5'HA-TriTag (mCherry)-3'HA (HDR donor)

Plasmid

#164047

Purpose

CRISPR donor plasmid to insert TriTag (mCherry) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mCherry-TriTag

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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JPF0455

Plasmid

#124040

Purpose

Encodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pPGK1-NES-tagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0456

Plasmid

#124041

Purpose

Encodes pPGK1 driving expression of mScarlet in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pPGK1-mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAGM16418

Plasmid

#105404

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

6xHis-NES(Rev)-GFP

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF511_1

Plasmid

#104044

Purpose

Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF511 gRNA (ZNF511 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF511_2

Plasmid

#104045

Purpose

Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF511 gRNA (ZNF511 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtK171S

Plasmid

#92404

Purpose

hQPRTase harbouring K171S mutation

Depositor

James Naismith, Huanting Liu

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Use

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

K171S

Promoter

T7

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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FH95pUP95S73DGW (F3)

Plasmid

#74040

Purpose

lentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusion

Depositor

Weifeng Xu, Robert Malenka, Bernardo Sabatini

Article

Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

Insert

Psd95

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

S73D

Promoter

H1

Available since

Availability

Academic Institutions and Nonprofits only
N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Article

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Use

Tags

8 histidine tag

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.b201

Plasmid

#131404

Purpose

E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody b201

Use

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-myc-GLUT4-mCherry

Plasmid

#64049

Purpose

3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

GLUT4 (Slc2a4 Mouse)

Use

Lentiviral

Tags

c-Myc and mCherry

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDN77

Plasmid

#61347

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-biNLS2 and NES IkBa

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHB2

Plasmid

#187797

Purpose

MAC-tagged gene expression of human EPHB2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHB2 (EPHB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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