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NEK9 gRNA (BRDN0001146898)

Plasmid

#75663

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK9

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK9 (NEK9 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GSG2 gRNA (BRDN0001145625)

Plasmid

#77520

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSG2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSG2 (HASPIN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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NEK9 gRNA (BRDN0001145225)

Plasmid

#76531

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK9

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK9 (NEK9 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shPIK3CD.v2 puro

Plasmid

#58707

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Hes3

Plasmid

#61535

Purpose

Expresses Hes3 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes3 (Hes3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pLG1-puro-sgATL2-2

Plasmid

#109009

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #2 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Enh eGFP Reporter-muKC2-X33

Plasmid

#59257

Purpose

Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

Depositor

Benjamin Yu

Insert

Krt81-ex4-7

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Mu Hsp68 minimal promoter

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
pFCK(1.3)tdimer2W

Plasmid

#27233

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

DsRed2

Use

Lentiviral

Tags

tandem dimer RFP

Expression

Mutation

Promoter

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-GFP-PGK-Puro

Plasmid

#110865

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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PRKACA gRNA (BRDN0001144986)

Plasmid

#75515

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKACA gRNA (BRDN0001145395)

Plasmid

#75516

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKACA gRNA (BRDN0001145877)

Plasmid

#75517

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKACA gRNA (BRDN0001148333)

Plasmid

#75518

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKACA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKACA (PRKACA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
IGF1R gRNA (BRDN0001146062)

Plasmid

#76691

Purpose

3rd generation lentiviral gRNA plasmid targeting human IGF1R

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IGF1R (IGF1R Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-Puro

Plasmid

#110848

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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MAPK15 gRNA (BRDN0001145787)

Plasmid

#75618

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK15 gRNA (BRDN0001148491)

Plasmid

#75619

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK15 gRNA (BRDN0001162376)

Plasmid

#75620

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPK15 gRNA (BRDN0001162374)

Plasmid

#75621

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PDK4 gRNA (BRDN0001148073)

Plasmid

#76438

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDK4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDK4 (PDK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge