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Plasmid#183322PurposeAll-in-One CRISPRko system with a guide RNA that targets SRC geneDepositorInsertSRC
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL2
Plasmid#183268PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL2 geneDepositorInsertABL2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL1
Plasmid#183267PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL1 geneDepositorInsertABL1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191183PurposeExpression of PPIB sensor in mammalian cellsDepositorInsertmCherry:PPIB.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-AD8gp160
Plasmid#123260PurposeMammalian expression plasmid for Env from the AD8 HIV-1 isolateDepositorInsertHIV-1 (AD8) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPI460
Plasmid#113204PurposeInducible expression vector (dox-on), C-terminal mScarlet taggingDepositorTypeEmpty backboneUseDictyostelium discoideum inducible expression vec…TagsmScarletExpressionMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)
Plasmid#191142PurposeExpression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.DepositorInsertmCherry:s17d9b:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-Rac1 5'-UTR
Plasmid#115354Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of Rac1DepositorInsertRac1 5'-UTR (RAC1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSV40 7xmutmiR-122T
Plasmid#35663DepositorInsert7 mutant miR-122 target sites
UseLuciferaseTagsExpressionMutationPromoterSV-40Available sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_VPR_EYFP
Plasmid#167890PurposePiggyBac compatible plasmid expressing Cas9-VPRDepositorInsertCas9-VPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NGFP-mAID
Plasmid#140605PurposeExpression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron-G
Plasmid#36887DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
UseTagsExpressionBacterialMutationPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJZC593
Plasmid#62319PurposesgRNA with MS2-PP7 for yeast cellsDepositorInsertsgRNA + MS2-PP7 RNA binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pJMP1054
Plasmid#119241Purposeconstruction intermediateDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1050
Plasmid#119240Purposeconstruction intermediateDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1210
Plasmid#91033PurposeModule A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSPDepositorInsertTaCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoterZmUbiAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0.062
Plasmid#119651PurposeLevel 0 part. Terminator for luxICDABEG operon from Vibrio fischeri.DepositorInsertBba_B0011
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_EYFP
Plasmid#167866PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only