Purpose
Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.
Insert
Twin Strep tag with RPL3 genome HomoArm (
RPL3 Synthetic, Human)
Use
CRISPR; Ssdna donnor preparation for knock-in of…
Tags
Twin Strep
Expression
Mutation
Two SNP (rs749555789, rs760194863) were introduce…
Available since
Jan. 9, 2024
Availability
Academic Institutions and Nonprofits only