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Plasmid#58007PurposeLocalization: Gap Junctions, Excitation: 555, Emission: 584DepositorInsertCx26 (Gjb2 Rat)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
USH1C_PDZ_1
Plasmid#103936PurposeProtein expression and purification of P73 PDZ1 domainDepositorInsertUSH1C (USH1C Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMflPT-o4
Plasmid#101315PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
pac resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflST-o4
Plasmid#101316PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
aadA1 resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDK promoter dCas9
Plasmid#113657PurposedCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.DepositorInsertTDK gene promoter and dCas9
UseCRISPRTagsExpressionBacterialMutationPromoterTDK gene promoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB012
Plasmid#119709PurposeComplete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMTDepositorInsertPtrpC:hph:Ttub
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos4a (mEosFP4a-V69T)
Plasmid#99225PurposeBacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).DepositorInsertpr-mEos4a
UseTagsExpressionBacterialMutationV69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVRa17_1691
Plasmid#49672DepositorInsertECF17_1691
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.4-Fc-His
Plasmid#72170PurposeExpresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema6d.4 (Sema6d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
M26I DJ-1 TEV site pET15b
Plasmid#60685PurposeRecombinant protein expression of Park7DepositorInsertM26I DJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationM26I and TEV site was inserted (see comments belo…PromoterT7Available sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-Amp
Plasmid#108704PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-Amp
Plasmid#108707PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-0-CFP
Plasmid#41664DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; C-terminal hyd…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE125 XDsh delta COOH-GFP-CS2+
Plasmid#16784DepositorInsertXdsh-delta-COOH (dvl2.L Frog)
UseXenopus expressionTagsGFPExpressionMutationcontains Dsh amino acids 1-588 (deleting 599-736)PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
AI-CN590
Plasmid#62596PurposeKCTD13 N-terminus disruption by homolgy-directed repairDepositorInsertCerulean-P2A-PuroR (KCTD13 Human)
UseTALEN; Homology-directed repair donorTagsExpressionMammalianMutationPromoterhEF1alphaAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.y-Fc-His
Plasmid#72181PurposeExpresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5c.y (Unc5c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Neuroserpin oloop
Plasmid#58260PurposeRetroviral vector for expressing mutated Neuroserpin in mammalian cellsDepositorInsertNeuroserpin (SERPINI1 Human)
UseRetroviralTagsExpressionMammalianMutationMutant of neuroserpin lacking five residues from …PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sema3e(S)-AP-His
Plasmid#72020PurposeExpresses the Sema3E protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3e (Sema3e Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5b.z-AP-His
Plasmid#72053PurposeExpresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertUnc5b.z (Unc5b Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.y-AP-His
Plasmid#72055PurposeExpresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertUnc5c.y (Unc5c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only