We narrowed to 22,396 results for: crispr

1. pRDA_151

   Plasmid

   #179084

   Purpose

   nuclease Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   Cas9-HF
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. C-1-BE

   Plasmid

   #135029

   Purpose

   C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   PmCDA1L1_4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. px330 EQR

   Plasmid

   #101733

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAT9749-dABE

   Plasmid

   #162998

   Purpose

   plasmid expressing ABE with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dABE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BlatCas9

   Plasmid

   #163792

   Purpose

   The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

   Depositor

   Yongming Wang

   Article

   Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAT15002-ABEmax

   Plasmid

   #163006

   Purpose

   plasmid expressing ABEmax

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   ABEmax
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAT15064-eCBE

   Plasmid

   #163009

   Purpose

   plasmid expressing eCBE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   eCBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAT9676-ABE

   Plasmid

   #162997

   Purpose

   plasmid expressing ABE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   ABE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCSDest2-SpCas9-NLS-3XHA-NLS

   Plasmid

   #69220

   Purpose

   Expresses wild type SpCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PABE-2

    Plasmid

    #115623

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBluescript_VEGFA-TS3_SaCas9_sgRNA2

    Plasmid

    #107330

    Purpose

    U6 driven SaCas9 sgRNA expression for VEGFA site 3

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    VEGFA-TS3 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EE-SP!gIII

    Plasmid

    #48667

    Purpose

    Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9
    anti-gIII tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    proC and tracrRNA promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SP_gRNA_pUC19_N_FancF_Left

    Plasmid

    #79892

    Purpose

    Human gRNA expression vector targeting FANCF (site 1 left)

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Insert

    FancF_Site_1_Left
    

    Use

    CRISPR

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPOR1CB0113

    Plasmid

    #117552

    Purpose

    Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. N-4-BE

    Plasmid

    #135019

    Purpose

    C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9

    Depositor

    Tian-Lin Cheng, Zilong Qiu

    Article

    Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

    Insert

    LjCDA1L1_4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 with EF-1alpha intron

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBSKΔB-CBh-Cas9n-pA

    Plasmid

    #82617

    Purpose

    Expresses Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    SpCas9n
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFH71

    Plasmid

    #128214

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. px458_2A_GFP_sgRNA_RNF123

    Plasmid

    #127122

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA RNF123 (RNF123 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPOR2KN0079

    Plasmid

    #117646

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116956

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge