We narrowed to 23,416 results for: crispr

1. MbCas12a_K487_GPG_GR2

   Plasmid

   #231577

   Purpose

   Encodes a cortisol inducible variant of MbCas12a

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   MbCas12a with a GR2 insertion behind K487
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSJ105

   Plasmid

   #208859

   Purpose

   Expresses SoxS activation domain and gRNA targeting 105 region within the synthetic promoter in bacterial cell

   Depositor

   Marc Erhardt

   Article

   Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

   Insert

   sgRNA 105
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   CRISPR/dCas9-responsive synthetic promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

   Plasmid

   #219823

   Purpose

   EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

   Depositor

   Philipp Maass

   Insert

   RfxCas13d
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core promoter

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pzk488-pmax-NES-PUFc-FseI[ADAR2-DD-splitN]

   Plasmid

   #196841

   Purpose

   Express NES-PUFc-FseI[ADAR2-DD-splitN]

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   NES-PUFc-FseI[ADAR2-DD-splitN]
   

   Expression

   Mammalian

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pzk491-pmax-NES-PUFc-FseI[ADAR2-DD-splitC]

   Plasmid

   #196842

   Purpose

   Express NES-PUFc-FseI[ADAR2-DD-splitC]

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   NES-PUFc-FseI[ADAR2-DD-splitC]
   

   Expression

   Mammalian

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ300

   Plasmid

   #205414

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter

   Depositor

   Yiping Qi

   Article

   Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

   Insert

   Ev1Cas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS (N terminal on insert) NLS (C terminal on ins…

   Expression

   Plant

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ301

   Plasmid

   #205415

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoter

   Depositor

   Yiping Qi

   Article

   Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

   Insert

   Hs1Cas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Expression

   Plant

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMB1-A-ptet-FLAG-dCas9

   Plasmid

   #190130

   Purpose

   Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRi

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-TcCas12b-T2A-EGFP

   Plasmid

   #188277

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

   Insert

   TcCas12b
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR-U6-gRNA-TRE3G-Nluc

    Plasmid

    #192659

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    U6, TRE3G

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR-U6-gRNA-miniCMV-Nluc

    Plasmid

    #192657

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    U6, miniCMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMLS1272

    Plasmid

    #188891

    Purpose

    Ric-4 sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(R07E5.16)::Ric-4 sgRNA (CELE_Y22F5A.3 Nematode)
    

    Expression

    Worm

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEcCas5

    Plasmid

    #170090

    Purpose

    expresses E. coli type I-E Cas5

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E Cas5
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEcCas7

    Plasmid

    #170092

    Purpose

    expresses E. coli type I-E Cas7

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E Cas7
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEcCas8

    Plasmid

    #170093

    Purpose

    expresses E. coli type I-E Cas8

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E Cas8
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEcCse2

    Plasmid

    #170094

    Purpose

    expresses E. coli type I-E Cse2

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E Cse2
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. EC0_6_sgRNA

    Plasmid

    #163717

    Purpose

    Plasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    sgRNA expression cassette
    

    Use

    Synthetic Biology; Moclo vector level 0 (type 234)

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC_cherry donor_Rosa target (pE002)

    Plasmid

    #149348

    Purpose

    Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMC_cherry donor_Polb target (pE038)

    Plasmid

    #149349

    Purpose

    Replace donor vector for cherry insertion using the human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJMP2373

    Plasmid

    #160079

    Purpose

    Z. mobilis CRISPRi, promoter F, empty guide, sfGFP

    Depositor

    Jason Peters

    Article

    Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

    Insert

    empty sgRNA
    

    Expression

    Bacterial

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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