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pET28:LanII-56

Plasmid

#225073

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-56)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-859

Plasmid

#218009

Purpose

mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCerulean

Use

Synthetic Biology

Tags

Factor Xa protease cleavage site and Strep-tag II

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-839

Plasmid

#217995

Purpose

sAP1 protein secretion signal

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Secretion signal peptide derived from the secretory protein secreted acid phosphatase 1 (sAP1)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-845

Plasmid

#218000

Purpose

Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Strep-tag II

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-849

Plasmid

#218004

Purpose

8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b C7H1G6_6H N-terminal

Plasmid

#223434

Purpose

C7H1G6 GNAT expression vector with N-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

C7H1G6 GNAT

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b Salmonella NAT_6H C-terminal

Plasmid

#223436

Purpose

Salmonella NAT expression vector with C-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

Salmonella NAT

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b R6CZ24_6H C-terminal

Plasmid

#223435

Purpose

R6CZ24 Thiolase expression vector with C-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

R6CZ24 Thiolase

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin XLone-eGFP

Plasmid

#159754

Purpose

AAVS1 donor plasmid for targeted inducible eGFP expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

all-in-one tet-on system

Use

CRISPR and TALEN; Donor plasmid for targeted knoc…

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS inducible promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP_KDEL

Plasmid

#221411

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP_KDEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP_myr

Plasmid

#221412

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP_myr

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP

Plasmid

#221413

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP_mito

Plasmid

#221414

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP_mito

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-SLC66A2_STOP

Plasmid

#221419

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC66A2 (PQLC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-TMEM144

Plasmid

#221426

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

TMEM144 (TMEM144 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Sensor_P2A_SLC2A4

Plasmid

#221429

Purpose

Gateway entry clone encoding a polyprotein that is cleaved to FRET glucose sensor and SLC2A4 proteins

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

Sensor_P2A_SLC2A4 (SLC2A4 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAP

Plasmid

#221891

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

TAF15 (TAF15 Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDV18 [punc-47::SNG-1::CRY2olig(535)]

Plasmid

#197598

Purpose

Expression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)

Use

Tags

Expression

Worm

Mutation

E490G

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDV12 [psng-1::SNG-1::CRY2olig(535)]

Plasmid

#197596

Purpose

Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)

Use

Tags

Expression

Worm

Mutation

E490G

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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ENTR_BN001T-N_START

Plasmid

#218058

Purpose

RESOLUTE SLC binder cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001T-N_START

Use

Cloning plasmid

Tags

Expression

Mutation

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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