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pUCBB-ptet-eGFP

Plasmid

#32554

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Mutation

Promoter

ptet

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)

Plasmid

#159886

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-1(no_atg)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

Plasmid

#159887

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-2 (no_start)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-TuDmiR122

Plasmid

#35669

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD miR-122

Use

Tags

Expression

Mutation

Promoter

U6

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR1

Plasmid

#183289

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR2

Plasmid

#183290

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_EPHA2

Plasmid

#183286

Purpose

All-in-One CRISPRko system with a guide RNA that targets EPHA2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

EPHA2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pPI420

Plasmid

#113230

Purpose

safe haven knock-in plasmid for the act5 locus, Expression of H2B-mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

H2B

Use

Safe haven knock-in plasmid for the act5 locus, h…

Tags

mCherry

Expression

Mutation

Promoter

act5

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC595

Plasmid

#62322

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Use

Tags

VP64

Expression

Yeast

Mutation

Has nuclease-inactivating mutations in the RuvC1 …

Promoter

Tdh3 and pAdh

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PDGFRB

Plasmid

#67130

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PDGFRB (PDGFRB Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCA0.002

Plasmid

#119560

Purpose

Level 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB6682

Plasmid

#106152

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

Plasmid

#168189

Purpose

HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4,K852A)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneoRL-BCL2 3'UTR

Plasmid

#84597

Purpose

Renilla luciferase reporter containing BCL2 3' UTR

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

BCL2 3'UTR (UTS2R Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mutation

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pCEP4-25711gp160

Plasmid

#111841

Purpose

Mammalian expression plasmid for Env from the 25711 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (25711.2) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101107

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-sGFP

Plasmid

#79508

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

superGFP

Use

Multisite gateway entry clone

Tags

6xHis

Expression

Mutation

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-myc-BioID-C

Plasmid

#174737

Purpose

Overexpresion

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PRKCA

Plasmid

#67115

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PRKCA Protein kinase C alpha type (PRKCA Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pUASTattb-cAMPr Y205A, R626A

Plasmid

#84932

Purpose

cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr Y205A R626A

Use

Tags

Expression

Insect

Mutation

PKA-C Y205A, PKA-R R626A

Promoter

5X UAS

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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