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Plasmid#194010PurposeFor CRISPR knockout of integrin alpha5DepositorInsertsingle-guide RNA (a5_guide A)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-26
Plasmid#160218PurposeAtpU6-26 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-26 promoter
UseGolden gate level 0 piece to express grnasTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-29
Plasmid#160219PurposeAtpU6-29 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-29 promoter
UseGolden gate level 0 piece to express grnasTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABE‐NG‐loop
Plasmid#187057PurposeABEmax-SpCas9-NG-loopDepositorInsertABE‐NG‐loop
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL237
Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L1-dSpRY
Plasmid#177681PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L2-dSpRY
Plasmid#177682PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)
Plasmid#181790PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15484_HF-ABE8e
Plasmid#174122PurposeExpresses ABE8e fused with SpCas9-HF1DepositorInsertHF-ABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSARN-vasa-eSpCas9sv40
Plasmid#173670PurposeeSpCas9 expression vector for AnophelesDepositorInserteSpCas9 with Anopheles vasa promoter and SV40 terminator
UseCRISPRTagsExpressionInsectMutationmutations in Cas9 reducing off-target activity (S…PromotervasaAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro
Plasmid#174118PurposeExpression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)DepositorInsertsgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
UseLentiviralTagsExpressionMutationPromotermU6 and UbCAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9992-HF-ABE
Plasmid#163000Purposeplasmid expressing HF-ABEDepositorInsertHF-ABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9994-HypaR661A-ABE
Plasmid#163002Purposeplasmid expressing HypaR661A-ABEDepositorInsertHypa-ABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationR661APromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9996-HeF-ABE
Plasmid#163004Purposeplasmid expressing HeF-ABEDepositorInsertHeF-ABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9748-dCBE
Plasmid#163008Purposeplasmid expressing CBE with dCas9DepositorInsertdCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330.puro sgRNA KGA Stop1
Plasmid#110405PurposeCRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistanceDepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Gal4-RNF169UBD
Plasmid#119013PurposeMammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expressionDepositorInsertGal4 (GAL4 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1052B (Ubiq-Fluc_Ubiq-Rluc)
Plasmid#132426PurposeExpress Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoterDepositorInsertsUbiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only