Skip to main content
Addgene
Showing: 11301 - 11320 of 13552 results

  2. pJK606

    Plasmid
    #72529
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subunit with C-terminal His6 tag (AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    72529-plasmid-map-sequence-id-132617

    Enlarge

  4. pJK290

    Plasmid
    #71554
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-39 to alanine and tyrosine-260 to …
    Promoter
    T7
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71554-plasmid-map-sequence-id-139946

    Enlarge

  5. pJK326

    Plasmid
    #71557
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-368 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71557-plasmid-map-sequence-id-134622

    Enlarge

  6. pJK325

    Plasmid
    #71556
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-367 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71556-plasmid-map-sequence-id-131415

    Enlarge

  7. pJK500

    Plasmid
    #72245
    Purpose
    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and endogenous
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72245-plasmid-map-sequence-id-140434

    Enlarge

  8. pJK345

    Plasmid
    #71559
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 and alanine-368
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71559-plasmid-map-sequence-id-134626

    Enlarge

  9. pJK344

    Plasmid
    #71558
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 (same as deleting alanine-368)
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71558-plasmid-map-sequence-id-134625

    Enlarge

  10. pJK624

    Plasmid
    #72539
    Purpose
    Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72539-plasmid-map-sequence-id-132631

    Enlarge

  11. pJK625

    Plasmid
    #72541
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72541-plasmid-map-sequence-id-132634

    Enlarge

  13. 4QQI

    Plasmid
    #61949
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. 2MJ8

    Plasmid
    #61956
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDYL2 (CDYL2 Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. 3HX4

    Plasmid
    #60137
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TgCDPK4:MAC03F-A01:C208088
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. 4LG6

    Plasmid
    #60093
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. L3MBTL1 (3P8H)

    Plasmid
    #28265
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL1 (L3MBTL1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 200-530.
    Promoter
    Available since
    April 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. CHKB (2IG7)

    Plasmid
    #25516
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CHKB (CHKB Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. ARL10B

    Plasmid
    #25522
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ARL10B (ARL8A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. CENTA1 (3LJU)

    Plasmid
    #25340
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CENTA1:K3-F370 (ADAP1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids K3-F370
    Promoter
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 11301 - 11320 of 13552 results