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pcDNA3-Q842gp120-8his

Plasmid

#100932

Purpose

Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Use

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pVRa11_987

Plasmid

#49661

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF11_987

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro BRIT1

Plasmid

#16206

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

shBRIT1 (MCPH1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCaSpeR4 cyto Grx1-roGFP2

Plasmid

#64999

Purpose

Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Use

Tags

roGFP2

Expression

Insect

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBEG R2-iNeo-L3 #BGV207

Plasmid

#48986

Purpose

Supplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Insert

neomycin resistance

Use

Gateway cloning vector

Tags

IRES (weak)

Expression

Mutation

Promoter

NA

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pXPR_sgGFP3

Plasmid

#119875

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

Plasmid

#48253

Purpose

LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-VP64

Use

AAV; Tale

Tags

2A_GFP

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 AK3 Hyg

Plasmid

#37539

Depositor

David Sidransky

Article

Chang et al PLoS One. 2011;6(6):e20806. Epub 2011 Ju

Insert

AK3 (AK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
ETV7_A86D_pet28a

Plasmid

#131657

Purpose

Bacterial expression of ETS gene with A86D mutation for solubility

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV7 A86D (ETV7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Alanine 86 to Aspartic Acid

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PLK3 1-334 T219D-pEGFP

Plasmid

#23268

Depositor

Wei Dai

Article

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

aa 1-334 T219D

Promoter

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
HOXD12 (murine) pRSETa

Plasmid

#8554

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXD12 (Hoxd12 Mouse)

Use

Tags

HIS and XPress

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-OTUB1 T134R,C91S-pcDNA3.1

Plasmid

#118212

Purpose

Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine and threonine 134 t…

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Intersectin I SH3 D domain (human)

Plasmid

#47416

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain D (ITSN1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain D

Promoter

tac

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
Intersectin I SH3 E domain (human)

Plasmid

#47417

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

ITSN1 intersectin 1 (ITSN1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain E

Promoter

tac

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pVRa41_491

Plasmid

#49702

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF41_491

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Psicheck2 MERTK full length 3'UTR

Plasmid

#26992

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

MERTK Full length 3' UTR (MERTK Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
px458_2A_GFP_sgRNA_PELO

Plasmid

#127124

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA PELO (PELO Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV FHA-Stop

Plasmid

#27050

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Mutation

changed glycine 141 to stop codon.

Promoter

Available since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only
pLKO-sh-Myocardin

Plasmid

#100769

Purpose

Lentiviral expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Lenti-sh-Myocardin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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B0032m_BC

Plasmid

#66020

Purpose

MoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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