Addgene

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H2B-GFP-Aurora B WT

Plasmid

#184042

Purpose

Expresses H2B-GFP fused to Aurora B WT

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

AURKB (AURKB Human)

Use

Tags

EGFP and H2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-T394A

Plasmid

#204994

Purpose

Expresses shRNA resistant GFP-RepoMan T394A Mutant

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell devision cycle associated 2 (CDCA2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed Threonine 394 to Alanin

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSD090A

Plasmid

#218132

Purpose

Hybrid circuit displaying mmCRBN bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

Crbn (Crbn Mouse)
luxAB

Use

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD138A

Plasmid

#218130

Purpose

Hybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Use

Tags

scP22

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD094B

Plasmid

#218131

Purpose

Hybrid circuit displaying mmCRBN bait and driving expression of gIII

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

Crbn (Crbn Mouse)
M13 pIII

Use

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM185A

Plasmid

#218126

Purpose

Hybrid circuit displaying hsCRBN bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Use

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Plasmid

#217512

Purpose

KD Recombinase dependent conditional smGdP-10XOllas cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Use

Tags

smGdP-10XOllas

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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HA-C Sars-CoV-2 E P71L

Plasmid

#194804

Purpose

Sars-CoV-2 E_P71L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

E_P71L (E Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

P71L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_D3L

Plasmid

#194806

Purpose

Sars-CoV-2 N_D3L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_N_D3L (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

N_D3L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_D63G

Plasmid

#194807

Purpose

Sars-CoV-2 N_D63G in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_D63G (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

D63G

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_R203M

Plasmid

#194810

Purpose

Sars-CoV-2 N_R203M in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_R203M (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

R203M

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_S235F

Plasmid

#194811

Purpose

Sars-CoV-2 N_S235F in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_S235F (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

S235F

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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HA-C Sars-CoV-2 N_T205I

Plasmid

#194812

Purpose

Sars-CoV-2 N_T205I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_T205I (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

T205I

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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LMPd Amt LDHA

Plasmid

#209408

Purpose

Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Kaymak et al Cell Metab. 2022 Sep 6;34(9):1298-1311.e

Insert

LdhA shRNA (Ldha Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PJT220 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF473 KRAB-T2A-mCherry-BSD-WPRE

Plasmid

#188759

Purpose

Lentiviral backbone containing ZNF473 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Insert

ZNF473 KRAB (ZNF473 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV4 lenti pEF-rTetR(SE-G72P)-3XFLAG-ANM2(SH3_1)-T2A-mCherry-BSD-WPRE

Plasmid

#188750

Purpose

Lentiviral backbone containing ANM2 SH3_1 domain as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Insert

ANM2 SH3_1 (PRMT2 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

Plasmid

#187344

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Insert

dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

KRAB WSR7EEE

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#187324

Purpose

Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Insert

BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Use

CRISPR and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

Plasmid

#187327

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Insert

dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

KRAB WSR7EEE

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge