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Plasmid#36114DepositorInsertG protein beta subunit B4 (Gnb4 Mouse)
UseTagsmcherryExpressionMammalianMutationPromotercmvAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCru5-/CGA-mEGFP-IRES-mCherry
Plasmid#49224PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pU6_(GLuc)_INT[P4-P6(3xPP7)]
Plasmid#68432PurposeTransient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
KA1874_pGL4-UBCmin-luc2/Sox2_SRR1
Plasmid#124197PurposeLuciferase reporter driven by the Sox2 enhancerDepositorInsertSox2 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2627_pGL4-UBCmin-luc2/Jam2-A'
Plasmid#124200PurposeLuciferase reporter driven by the Jam2 enhancerDepositorInsertJam2 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1867_pGL4-UBCmin-luc2/Pou5f1_CR2
Plasmid#124193PurposeLuciferase reporter driven by the Pou5f1 enhancerDepositorInsertPou5f1 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1866_pGL4-UBCmin-luc2/Pou5f1_CR3
Plasmid#124192PurposeLuciferase reporter driven by the Pou5f1 enhancerDepositorInsertPou5f1 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1865_pGL4-UBCmin-luc2/Pou5f1_CR4
Plasmid#124191PurposeLuciferase reporter driven by the Pou5f1 enhancerDepositorInsertPou5f1 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1403_pGL4-UBCmin-luc2/Nanog-B'
Plasmid#124190PurposeLuciferase reporter driven by the Nanog enhancerDepositorInsertNanog enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1402_pGL4-UBCmin-luc2/Nanog-A'
Plasmid#124189PurposeLuciferase reporter driven by the Nanog enhancerDepositorInsertNanog enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 12
Plasmid#36109DepositorInsertG protein subunit Gamma 12 (Gng12 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SIK1
Plasmid#51577PurposeConstitutively express nucleolus localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSIK1 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 13
Plasmid#36110DepositorInsertG protein subunit Gamma 13 (GNG13 )
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-SNF7
Plasmid#51574PurposeConstitutively express endosome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSNF7 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.miR16
Plasmid#128747PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.mutant
Plasmid#128746PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutation5 point mutations disrupting the miR-7 sitePromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.5
Plasmid#158407PurposeExpress single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only