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pEGFP-N1-2x

Plasmid

#172282

Purpose

Expressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-2x

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-4x

Plasmid

#172283

Purpose

Expressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-4x

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-1-CmYLCV-6

Plasmid

#197699

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

CmYLCV promoter

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-RLuc-9

Plasmid

#197739

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Renilla luciferase (tomato optimized)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

Plasmid

#191151

Purpose

Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1L72:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe (FA)

Plasmid

#170626

Purpose

For the mammalian expression of mLOV2GIVe GEF-deficient mutation F1685A

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (FA)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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VV201: ChR2(C128S)-eGFP in fubi

Plasmid

#58510

Purpose

Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

ChR2(C128S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Cysteine 128 to Serine

Promoter

Ubiquitin

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pMOD_A3701

Plasmid

#91052

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-dAtCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191182

Purpose

Expression of EACTB sensor in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:ACTB.s1:EGFP-3xMS2

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191184

Purpose

Expression of RPLP1 sensor in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:RPLP1.s1:EGFP-3xMS2

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

Plasmid

#191175

Purpose

Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (frameshifted)

Use

Tags

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 IL2

Plasmid

#67053

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL2 (IL2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pBDK

Plasmid

#135190

Purpose

Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

KanR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

cat

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJB045 (ZapB-mEos2)

Plasmid

#72651

Purpose

Inducible expression of ZapB-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapB-mEos2

Use

Tags

mEos2

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Flag Snail 4SA

Plasmid

#16220

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

4SA: S108A, S112A, S116A, S120A

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pAID-CMV linearizing in pX330

Plasmid

#140609

Purpose

Crispr/Cas9 plasmid for linearization of pAID-CMV

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

sgRNA for pAID-EF1a plasmid digestion

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB6677

Plasmid

#106155

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pME-Trpa1b-2A-mCherry

Plasmid

#106291

Purpose

Gateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherry

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Insert

Trpa1b (trpa1b Zebrafish)

Use

Gateway middle entry

Tags

2A-mCherry

Expression

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-Renilla

Plasmid

#79496

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Renilla Luciferase

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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