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Plasmid#57629PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertKeratin (KRT18 Human)
UseTagstdEosExpressionMammalianMutationV282LPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
FRIG-myc-Sema4D-CD4
Plasmid#51606Purposeexpresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backboneDepositorInsertSema4D/CD4-FLAG (Sema4d Human, Mouse)
UseLentiviralTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterRSVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-K74A K75A
Plasmid#49253PurposeMammalian expression of flag-tagged human Med26-K74A K75ADepositorInsertMed 26 (MED26 Human)
UseTagsHAExpressionMammalianMutationK74A K75APromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry
Plasmid#225588PurposeMammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2(S54L+T127A)-T2A-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry
Plasmid#225590PurposeAAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2 (S54L+T127A)-T2A-mCherry
UseAAVTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry
Plasmid#225584PurposeMammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2 (S54L+T127A)-T2A-mCherry
UseTagsmCherryExpressionMammalianMutationT21R-F8.2 (S54L+T127A)PromoterCMVAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG
Plasmid#222852PurposeMammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 FcDepositorInsertAT118-L
UseTagsIgG1_FcExpressionMammalianMutationL234A, L235A, P329GPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG
Plasmid#222854PurposeMammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 FcDepositorInsertAT118-L
UseTagsIgG1_FcExpressionMammalianMutationL234A, L235A, P329GPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
IL2-AHDC2-Cherry in pmCherryN1
Plasmid#187291PurposeExpress pmCherry-IL2-AH-deltaC2DepositorUseTagsmCherryExpressionMammalianMutationAH-deltaC2PromoterCMVAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-CD59
Plasmid#222307PurposeSynchronize the trafficking of CD59 from the ER.DepositorInsertStreptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)
UseTagsExpressionMammalianMutationsignal peptide removedPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-MCRS1
Plasmid#231241PurposeFlag-tagged wild-type MCRS1 over-expression construct for transient expression in mammalian cellsDepositorInsertMCRS1 (MCRS1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE
Plasmid#229544PurposeLentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion proteinDepositorInsertOct4(co)-mNeonGreen (POU5F1 Human)
UseLentiviralTagsOct4-mNeonGreenExpressionMammalianMutationcodon-optimized OCT4PromoterTRE12Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC
Plasmid#229545PurposeLentiviral construct to express rtTA and SKM proteinsDepositorInsertrTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhEF1aAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
hTln1-R12DD-SCLS
Plasmid#191443Purposeexpresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397DepositorInsertHuman Talin1 R12DD (TLN1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationdeletion between residues K2375 and A2397PromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_rPD-L1nb
Plasmid#226715PurposeHeterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
UseTagsHA tag and Histidine tagExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_GLuc
Plasmid#226718PurposePlasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)DepositorInsertGaussia Luciferase
UseTagsHA tag and Mating factor alpha secretion signalExpressionYeastMutationPromoterpTEF1Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC131: pAAV.CMV-CasRx-VEGFA sgRNA array
Plasmid#203444PurposePlasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAsDepositorInsertsU6-VEGFA sgRNA array
RfxCas13d
UseAAVTagsHA and NLSExpressionMammalianMutationPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM148: pAAV.CMV-Cas13e-NT sgRNA
Plasmid#203446PurposePlasmid expressing active RfxCas13d with non-targeting gRNADepositorInsertsU6-non targeting (NT) sgRNA
Cas13e
UseAAVTagsHA and NLSExpressionMammalianMutationPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM149: pAAV.CMV-Cas13e-VEGFA sgRNA
Plasmid#203447PurposePlasmid expressing active Cas13e with VEGFA mRNA targeting gRNADepositorInsertsU6-VEGFA sgRNA
Cas13e
UseAAVTagsHA and NLSExpressionMammalianMutationPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA
Plasmid#203451PurposePlasmid expressing active and codon optimised Cas13e with non-targeting gRNADepositorInsertsU6-non targeting (NT) sgRNA
Cas13e
UseAAVTagsHA and NLSExpressionMammalianMutationCodon optimised using GenScript toolPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only