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YFP Gamma 3

Plasmid

#36103

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 3 (Gng3 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
V5-WT-short-UBASH3A

Plasmid

#192088

Purpose

Mammalian expression of human wild-type short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3a

Plasmid

#59420

Purpose

Expresses lncRNA MEG3 isoform a in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3a (MEG3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Stephen Lory, Cammie Lesser

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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KA1870_pGL4-UBCmin-luc2/Esrrb-B'

Plasmid

#124196

Purpose

Luciferase reporter driven by the Esrrb enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pWPT-/CGA-mEGFP-IRES-mCherry

Plasmid

#49233

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Mutation

Promoter

EF1-short

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Claspin-Flag

Plasmid

#136246

Purpose

Protein expression of Claspin with a C terminal FLAG tag

Depositor

Aziz Sancar

Article

Sar et al J Biol Chem. 2004 Sep 17;279(38):39289-9

Insert

Claspin

Use

Tags

Flag

Expression

Mammalian

Mutation

E263G, Q499R, I783T, D932G and S1062G- Please see…

Promoter

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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KA1869_pGL4-UBCmin-luc2/Fgf4-A'

Plasmid

#124195

Purpose

Luciferase reporter driven by the Fgf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Fgf4 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-dKeNLuc_SV40:PuroR

Plasmid

#183046

Purpose

Expresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

dKeNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-KaNLuc_SV40:PuroR

Plasmid

#183047

Purpose

Expresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

KaNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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p-miR-148a

Plasmid

#65052

Purpose

Expression of miR148a in mammalian cells

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

hsa-miR-148a (pre-miRNA) (MIR148A Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pU1/Sm/3' Box_(GLuc)_INT

Plasmid

#68438

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-Bex2

Plasmid

#131607

Purpose

expression of mouse Bex2 in mammalian cells

Depositor

Filipe Pereira, Kateri Moore

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Bex2 (Bex2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F2

Plasmid

#184164

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F1

Plasmid

#184163

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pC-KIT2

Plasmid

#118984

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

mutK2-SP-K1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT4

Plasmid

#118986

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

K2-SP-mutK1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT6

Plasmid

#118988

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Use

Tags

Expression

Mammalian

Mutation

mutK2-SP-mutK1

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 3

Plasmid

#36113

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B3 (GNB3 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

cmv

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pCru5-/GCCACC-mEGFP-IRES-mCherry

Plasmid

#49226

Purpose

Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Retroviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Mutation

Promoter

Viral LTR (or CMV)

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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