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Plasmid#169090PurposeMoClo level 1 35S promoter-BvDODA1-Ocs terminator transcription unitDepositorInsert35S promoter-BvDODA1-Ocs terminator
UseMoclo cloning vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM7535
Plasmid#169089PurposeMoClo level 1 35S promoter-BvCYP76AD1-Nos terminator transcription unitDepositorInsert35S promoter-BvCYP76AD1-Nos terminator
UseMoclo cloning vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA M3
Plasmid#80936PurposeExpresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.MCV.LT339.V5.4
Plasmid#28193DepositorInsertMCPyV Truncated Tumor Large T antigen (399 tumor strain)
UseTagsV5ExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261
Plasmid#171429PurposeExpression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cellsDepositorInsertFKBP-(GGS)X6-HaloTag C261
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPI458
Plasmid#113212Purposesafe haven knock-in plasmid for the act5 locus, C-terminal mScarlet taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, c…TagsmScarletExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVPSB_empty
Plasmid#192170PurposepVPSB backbone expression vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x6
Plasmid#58776PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0908
Plasmid#91027PurposeModule A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSPDepositorInsertTREX2-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterEC1.2Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pconst #1-RFP
Plasmid#81075Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23119Available sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDM1514
Plasmid#108999Purposesafe haven knock-in plasmid for the act5 locus, N-terminal mCherry taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, n…TagsmCherryExpressionMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb719-HO
Plasmid#141200PurposeExpresses mRhubarb719 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb719
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, Y198S, T202Y, V203I, W309R, Q310A compared…PromoterT7Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-CmCherry-mAID
Plasmid#140604PurposeExpression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP
Plasmid#199456PurposeExpression of stdPCP-PH in mammalian cellsDepositorInsertstdPCP-PH
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZA-mCherry-R5
Plasmid#106258PurposeExpresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertmCherry
UseTagsAdded silaffin R5 peptide to C terminus of mCherr…ExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET14_GFP5_Encap
Plasmid#86408PurposeExpresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 5aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x4
Plasmid#58774PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1-Ni
Plasmid#17744DepositorInsertPIK3R1-Ni (PIK3R1 Human)
UseTagsExpressionInsectMutationPartial cDNAPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-2X snRP1
Plasmid#99688PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only